DreINT0115016 @ danRer10
Intron Retention
Gene
ENSDARG00000079585 | pkn3
Description
protein kinase N3 [Source:ZFIN;Acc:ZDB-GENE-030131-1902]
Coordinates
chr5:31238876-31239252:-
Coord C1 exon
chr5:31239109-31239252
Coord A exon
chr5:31238952-31239108
Coord C2 exon
chr5:31238876-31238951
Length
157 bp
Sequences
Splice sites
5' ss Seq
GAGGTGAGA
5' ss Score
7.66
3' ss Seq
ATGGTGTGGTTTGCTATCAGGTG
3' ss Score
7.06
Exon sequences
Seq C1 exon
TCTCGAGAACTGGAGATTGCTGTGTATTGGCGAGACTGGAGGTCCCTGTGTGCCGTGAAGTTCCTGCGTTTGGAAGACTTTCTAGACAACCAGAGGCACGGCATGTGTCTTTATCTGGAGCCGCAGGGCACACTGTTCACAGAG
Seq A exon
GTGAGAGAAGCTCTCAACACCTTCATCCTAGTGTCAAACCATGCGTGGTGTGCTTACTGTGTACAGCTGCTCTCTGTTTCTTTTAGACTTCGTAGATTCACTTAAAGCTTGAAAACCTAATTCATATACAGCTGTTTATGGTGTGGTTTGCTATCAG
Seq C2 exon
GTGAGATTCATCAATCCTGTCATTGAGCGGCATCCTAAACTACAACGACAGAAGAGGATTTTCCCTAAAGAAAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079585:ENSDART00000112546:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.154
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTCGAGAACTGGAGATTGCTG
R:
TTTTCTTTAGGGAAAATCCTCTTCTG
Band lengths:
218-375
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]