DreINT0124566 @ danRer10
Intron Retention
Gene
ENSDARG00000079872 | rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:ZFIN;Acc:ZDB-GENE-070912-385]
Coordinates
chr9:2958017-2960032:-
Coord C1 exon
chr9:2959902-2960032
Coord A exon
chr9:2958155-2959901
Coord C2 exon
chr9:2958017-2958154
Length
1747 bp
Sequences
Splice sites
5' ss Seq
CAGGTCTCC
5' ss Score
0.85
3' ss Seq
CGTGCACTTATCACGCTCAGGCG
3' ss Score
3.87
Exon sequences
Seq C1 exon
ATCCTGCTCTGGATGACTTTCTGCTCATGCACTGTGTTTTTGTCCCGAACAGTCAGCTGTGTCCACTGCTCATGACCCAATATCCTTCTTTAATAAAGCCCTACTGCTTTTCAACAAAAACACCACATCAG
Seq A exon
GTCTCCAGAAACCCTGCTGTAAATTCCAGCTTAAACCAGCCTAAGCTGCTTGGCTGGTTTTAGCTGGTCAGCCAGCCTGGTTTTAGAGGGGTTTTGGCCATTTCCAGGCTGGTTTCCAGCCATTTCCAGCCTGGTGTTAGCTGGTCAGGCTGGAAAATGACCTGCTAAAACCAACTAAAACTAGCTTGACCAGCCTGGTTTAAAGGGCACATAGGTTACCCCTTTTTTCATATTTATTATAAGTATTTTTAAAGTTTTAAAGGAAAGTTTCAGCTCAAAACACCCATCAGAATATTTATTAGAGCTGTTTGAAGTGTCTGTATTATAGCTGGGAAAAGGTTGTAGCTGTTTTTTTGCACTGGGCCTTTAAGGCGAGTCATCCCCGCCCACCAATCCCACGTGCCTGTCAGCAACAAGCCTCAATCGCCGCCCTCGGCTGCCTCAGGAAGCAGATCTCACGTAACGTTTGTGAGAAATACCACAGTAAGAACTTCACCAATCAGTATTTGATATCCCTGCTGAAAAATCCAACTTAAACCAGCCTAGGCTGGTTTAAGCTGGATTTTTCACCAGGGATGCATTTTTTGTGGAGTTGCAACAATGATTTCACGCACACACAGCTCAGACACGCAGACACACACACACACACACATGGCACATACACGTAGCATGACACATACGCACATACATAGCGTAGACACACAGGCAGACACATGTGCGCATGTTTTAAAACATGTTAAACTTGTAAAACTCACTCTTGATCACGTCTGATGATGATTGATGATCCTAGCAAAATGAACAGACCTTTTATTCCCGGTTGCTTTGCGCTCGTCCTGTCTTGTCGATATGATTATATGCATTACTATGGAGACATGTTAATGCGCGCAGCTGTCAATCAATATTGGTGGGCAGCGGGACCGCACTCCTACGTAAAGTCGCGGTTGATCTGAAAACCGCATCAATTGGTCTACCGTATTTATGTGGTAAAATAAAAAAAAAGCACTGTGTGTGTTTATTTCACCCCAATATGACAGTATATACACTATACCTACACACATGTCTGTCCAAACAGCTTGAAAAGTAGATTTTTCACCATAGGTGCCCTTTAAGATGGACACAGCTGGTTTTAGCTGGGCTCCCAGCCTGGTTAGGCTGGTCAAGCTGGTGTTAGCTGGTCATCTCCCAGCCTGACCAGCTAACACCAGGCTGGAAATAGCTGGAAACCAGCCTGGAAATGGCCAAAACCCCTCTAAAACCAGGGTGGTCAACCAGCTAAAACCAGCCAAGCAGCCTATGCTGGTTTAAGGTGGTTTTTTCAGCAGGAAAGAAAAATAAATGTTTATTTATTTAACCTCTGGCCATCAAAGATATACAGTTGAAGTCAGAATTATTAGCCCCCCCGTATATTATTTTCCCCCAATTTCTGTTTAACGGACTGAAGATACATAATAGTCTTAATAACTCATCTCTAATAACTGATTTATTTAACTTTGCCATGATGACAGCACATATTATTTGACTAGATATTCTTCAAGATACTAGTATTCAGCTTAAAGTGGCTTAACTAGATTGAATAGGCGAACTAGGGTAATAAGGCAAGTCATTGTATAACAGTGGTTTGTTCTGTAGACAGTCCAAAGAGCACGTTGTCTTCCTAAGTAAGTCTTTAACGAATAAGAGAGTCGCTGAACCCATAGTTTGTGCTTGTGTTTGAAGTCCTTAGCGCTCGTGCACTTATCACGCTCAG
Seq C2 exon
GCGTCCCAGGGCAGTGAACAGGAGCGGATGGATTACACTCTCAATAACAAGCGCAGGGTGGTGCGTCTGGTGTTACAGTGGGCCTCCATCAACACGGACCACCTGCAGGAGGAGGACAGCTCCTTCAGCTTCATACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079872:ENSDART00000144046:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.068 A=NA C2=0.217
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=FE(36.1=100)
A:
NA
C2:
PF0061815=RasGEF_N=FE(42.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTGCTCTGGATGACTTTCTGC
R:
CTGTCCTCCTCCTGCAGGTG
Band lengths:
248-1995
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]