DreINT0124792 @ danRer10
Intron Retention
Gene
ENSDARG00000036257 | rasal2
Description
RAS protein activator like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-180]
Coordinates
chr2:35855994-35856931:+
Coord C1 exon
chr2:35855994-35856138
Coord A exon
chr2:35856139-35856694
Coord C2 exon
chr2:35856695-35856931
Length
556 bp
Sequences
Splice sites
5' ss Seq
TTGGTAACT
5' ss Score
4.27
3' ss Seq
TTCAACTGTAATCCGGCCAGTGT
3' ss Score
3.99
Exon sequences
Seq C1 exon
GGGAGTTTATTAAAGCCCTGTACGAGTCGGATGAAAATTGTGAAGTAGACCAATCAAAGTGCACCAGCAACGAGCTTCCAGAGCATCAGAGTAACCTGAAGATGTGCTGCGAACTGGCCTTCTGCAAGATCATCAACTCTTATTG
Seq A exon
GTAACTTTTTTTTTTCATTTTTAAAATAATACAACAAAAATTAGTGAATTTATGTGAAGCATAAAACATATACACTATAATTCAAATGTTAATTTGGTTGTAGCATAAATTAATACAATTAATCTGATATACGTTTCAAATATCGGCAATTTTTTTGACTTGGCATAAATTCTTTTGTGTTTCTGTTGGTCAAAAAATATTGAAGAAAACATCTTTCCACAAAAATAATAAGTAGCACAACATTTTTAAACTTTGATCAAATTGAGAAAGTTATTTCAGCAGCAAATCATATAACCTATATAATATAATAATAGGTCTGTCATCACAAGAACAGTTATTTTCTATTGTAATCAAATTTCACTGTTGTATTTTTCATGATTAAATGTCTCATTTGTTTTGGATTTTTCAAAAACATTGTTTCAACAATAAACCTTTTGCCAGGGGAATTATGCCAATCATAATGCTTCTGCTGACAACTTCAAATACTGTCCTTGTTTGTAGTTTGACCATGCAAATGCATTCGTTCCATACTAACCTTCAACTGTAATCCGGCCAG
Seq C2 exon
TGTTTTTCCACGTGAACTGAAGGAGGTCTTTGCATCATGGAAGCAGCAGTGTCACGCTCGAGGGAAGCAGGACATCAGTCAGCGTCTTATCAGCGCATCGCTCTTCCTGCGCTTTCTGTGTCCTGCCATCATGTCCCCGTCACTCTTTGGTCTCATGCAGGAATATCCAGATGACCGTACTTCTCGAACACTCACCCTCATTGCCAAAGTCATTCAGAATCTTGCTAACTTTGCCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000036257:ENSDART00000113489:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=FE(27.9=100),PF067666=Hydrophobin_2=PU(46.9=46.9)
A:
NA
C2:
PF0061614=RasGAP=PD(44.2=95.0),PF067666=Hydrophobin_2=PD(51.0=31.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAAGCCCTGTACGAGTCGGAT
R:
TTTGGCAATGAGGGTGAGTGT
Band lengths:
342-898
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]