DreINT0124806 @ danRer10
Intron Retention
Gene
ENSDARG00000036257 | rasal2
Description
RAS protein activator like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-180]
Coordinates
chr2:35854622-35855883:+
Coord C1 exon
chr2:35854622-35855176
Coord A exon
chr2:35855177-35855738
Coord C2 exon
chr2:35855739-35855883
Length
562 bp
Sequences
Splice sites
5' ss Seq
AAGGTACGA
5' ss Score
9.78
3' ss Seq
GACTAAACTGTCTATATTAGGAC
3' ss Score
6.46
Exon sequences
Seq C1 exon
GATAATTGCCGGCGGGCGGAAAACGTCCTCCGCCTGTGGATCATTGAGGCAAAAGACCTGCCACCAAAAAAGAAATATTTCTGTGAGCTGTGTCTGGATGACATGCTGTACGCTCGTACCACCAGCAAAACCCGCTCCGACTGCCTGTTCTGGGGGGAACACTTTGAGTTTTCAGGCCTCCCGTCCATGAAAAGCATCACCATACACATCTACCGGGATGTGGACAAGAAAAAGAAAAAGGACAAAAACAACTATGTTGGCCTGGTGAACATCCCTGTGCATGGGGTGATGGGTAGGCAGTTTGTGGAGAAGTGGTATCCAGTCAGCACTCCCACCACCAGCAAAGCCAAAGGTGGAGGCCCCTCCATCCGCATCAAATCCCGCTTTCAGACCATCTCAATTCTGCCCATGGAGCAGTACAAGGAGTTTGCGGAGTTTGTTACCAATAACTATACCATGTTGTGCTCTGTGTTGGAGCCGGTCATAAGCGTGAAGAACAAAGAGGAGATGGCCAGCGCTCTGGTGCACATTCTGCAGAGCACGGGACGGGCAAAG
Seq A exon
GTACGATGCTAGTTAAAGTCCCATGAAGCCAATATTTCTTTGTTTTTTGGGGTGTTAGTATAGATTTTTTGTTGTGATATTGTCTACAAGCTAGTGTGCTACCAATAATTACATGAGAGGAAACAAAAGCATTTGAAACTTTACTTCATACTTTTTCTCATCTCAACTTCGAACAATCAAATGCTCTCTAGTAGCTGAAGTGTCCTCACCCCCTTCAAAAAGTGTTGCTGCATCTGAAATTAGCCATTTATTCATATGTACCTGAGCATTCATCGTGAAGGGTTCTCATAAACACTTAATGAGCAATTACTAGCCAGTAGCTTAATCCTCAATAAGTGGAATCTCAAAATGGCTGTGTGTTTACAGCAAGTCCAGAGGGGCAAGATACATTGCACATAAAAATATACTTTTAACAAACAGTAAACTAGGACAACTAGGTTTTCCTGTTTCAGCTGAATTTTCATTGCACGTTGAATAATCTGCTAATTTCAAGGCACTTCAAGGGACCTTTAAGTTTTTTGTTAACCATAATCACCAAAGTTGACTAAACTGTCTATATTAG
Seq C2 exon
GACTTCCTAACGGATTTGGTGATGTCAGAAGTGGATCGCTGTGCTGACCATGATGTGCTGATCTTTAGAGAAAACACACTAGCCACCAAAGCCATTGAAGAATACCTCAAACTGGTGGGACAGAAGTACCTACATGATGCACTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000036257:ENSDART00000113489:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.011 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0016825=C2=WD(100=43.8)
A:
NA
C2:
PF0061614=RasGAP=PU(27.3=95.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGTGAACATCCCTGTGCATG
R:
AGCACATCATGGTCAGCACAG
Band lengths:
352-914
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]