MmuINT0132047 @ mm10
Intron Retention
Gene
ENSMUSG00000070565 | Rasal2
Description
RAS protein activator like 2 [Source:MGI Symbol;Acc:MGI:2443881]
Coordinates
chr1:157174143-157176217:-
Coord C1 exon
chr1:157175663-157176217
Coord A exon
chr1:157174288-157175662
Coord C2 exon
chr1:157174143-157174287
Length
1375 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAGT
5' ss Score
11
3' ss Seq
ACTGAACATTATTCTTCCAGGAT
3' ss Score
6.33
Exon sequences
Seq C1 exon
GACAATTGTAGACGAGCTGAAAATGTTCTCCGTTTATGGATCATTGAAGCCAAGGACCTTGCCCCTAAAAAAAAATATTTCTGCGAACTGTGCCTTGATGATACCCTCTTTGCTCGTACAACCAGCAAGACCAAAGCAGACAATATTTTTTGGGGTGAACATTTTGAATTCTATAGCCTTCCACCTCTTCATAGCATCACGGTTCATATTTATAAGGATGTGGAAAAAAAGAAAAAAAAGGACAAGAATAATTATGTAGGGCTAGTCAATATCCCCACTGCCAGTGTGACTGGTCGCCAGTTTGTAGAAAAGTGGTACCCAGTGAGTACACCCACACCCAACAAAGGAAAGACAGGAGGACCTTCTATTCGGATTAAGTCACGTTTCCAAACTATCACCATTCTGCCTATGGAGCAATACAAAGAGTTTGCAGAATTCATCACCAGCAACTACACCATGCTGTGTTCTGTTCTTGAGCCAGTAATTAGTGTGAGGAATAAAGAGGAGTTGGCTTGTGCCTTAGTACACATTCTTCAAAGTACTGGCAGAGCCAAG
Seq A exon
GTAAGTCAAAAGGGGTATGTTACCTTAAAGTCCTTCCTTACCATGTGCAAAGAAAATGAAGCTGTGGACACTAAGAGACTGTGAAAGTCCAGTCGAAAAACTTCAGAAAAAGAAAAGGGAATTAAGGATGAGTTAAGAAAGCCTGTTTGCAGATCTGTAAGAGCTTGAGATTTTTAACTAAAATGAAATGGGAGAACTAGCAAACACTTATTTCTGGTTTGTTTCCATGAGGTAGGTAACATGGATGCAGTTGCTATAATAACCCCACAACATATTCAAATTCTGGTGTTATTGTTGTTTTTGTTCTTGTTTTCTAACCATGTTACCCATTCTAGCCCCAAACTTGTGATCTTCCTGCCTCAGCCTCCCAAATCCCAGAATTAAAGGGATTACAGATGTGCAACATCACGCCTAGCTAAAGTTTTTCTGGAGTCTTTAATCTTTCTGGAGTACTTTCTCAGTGTATTTTAAGATTAACTTCTAACTGTATCCCAGTGGCATAGAAGGAGATATGCAAGAAATGTACCAAGTGTAAATCTTCATGGAATTCAAATCTAGATCTGAAATGAAAACGTCAAGGGGTGTTGTACAGTTACTTGGCAGCTGTTTCATAGTAATCTTTCCATGTAAATGGAAATGAAAACTGGGAAGATACAGTAACACCCAGCAAGTGCCCGTAACTGTTAGGTGCTGGCGTGTGTCTCACTGTGTGTGTTGGACAAAAACAAACACTCTCTTTGACCTCCTGGAGCTCTCAGTTCTCTGAAGGACGATACTAAGTAATCGAAGAGTAAATCTGTGGCTATAGTGAAAAGTACACTGAGGAAAATGATAAACGGTTCTATGGAAGTGTGGAGAGGGGGAACGGGCAGGTGAGGTGCTGGGGTAATCCTGGTCCAGGAGGAAACACTGTGTGAGCTGAGATCTAAAGGGTTCCAGAAATTCATTTTATGGTATGATAGAGGTGAAGGTGGGAGGAGAGCAGAACTGAGAACAGCATTTGCAAAGGTTTCTTGTGGTGAATAGGAAGAGTTGTGTGTAGAGAAGGCTGTGTGGACCAGAGGACAAAAGAACCAGAGCCGAGAGGTTAGCATTAGGTATGGTGAGGGCAGGGCAGGGCAGGGGAAGAGAACTTGGAGTTGCATTCAGTAATTTTTTCAAAAGTGCATTAGAAAATCTCTGAAGGAATTCAGAACATAAATTGAGAGCTGGCATTACATAAGCTTTTTGTTCTGTCTGCCTGTTCTCTTTCCATGGAATTTGGTGACTTAGTTTTAAAGCAGTGTTCCCAGGATATCTGCTGTTTCCATAGTCTTTTCTAATGTTTGTTGCTTAACTTAGTTGCTTTCTTTCTGACTGAACATTATTCTTCCAG
Seq C2 exon
GATTTTCTGACTGACTTGGTGATGTCAGAGGTAGATCGTTGTGGAGAACATGATGTCTTGATCTTCAGAGAAAACACCATTGCCACTAAATCCATTGAGGAATACCTCAAGTTGGTGGGACAACAGTACCTTCATGATGCACTCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000070565:ENSMUST00000078308:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.042 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0016825=C2=WD(100=43.2)
A:
NA
C2:
PF0061614=RasGAP=PU(27.3=95.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACCCACACCCAACAAAGGAA
R:
GTTGTCCCACCAACTTGAGGT
Band lengths:
351-1726
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types