DreINT0124809 @ danRer10
Intron Retention
Gene
ENSDARG00000089933 | rasal3
Description
RAS protein activator like 3 [Source:ZFIN;Acc:ZDB-GENE-101130-3]
Coordinates
chr6:8239010-8239823:-
Coord C1 exon
chr6:8239679-8239823
Coord A exon
chr6:8239247-8239678
Coord C2 exon
chr6:8239010-8239246
Length
432 bp
Sequences
Splice sites
5' ss Seq
AGAGTAAGA
5' ss Score
5.73
3' ss Seq
TCTCACCTGCCTGCAAACAGGTC
3' ss Score
9.22
Exon sequences
Seq C1 exon
GTGACTTTATAGCAAGACTGTACAATGGAGGAGAGAACTGTGAGGTAGACCCGCTAAAATGCTCGCCCTCTGATCTACCAATCAACCAGAGACATCTTAAAGAGACTTGTGAGGATGTTGTGAAAAGAATCACTGACATGCAAGA
Seq A exon
GTAAGATCAACCTAAATGGACACACACACTACAAAGTATATTATAATAAATAGTGATAGAACAGAATAGGGGTGTAATGATATTCAAACTGAACCAAAAAAAAAAAAAACAGCATATGCCCTCTACGGTTCAAACCACAATTCTCTCATAGCAAACTGATGTTCAACCTCCCATCCACCCACCAGTCCCCCTCACTGAGTCCCCACATCCCTATCTGCTGTCTGCCATCCCATACACGTCCATTTTCACATTTTCGTCTATGCCCGGTTAATTGGATTGTTTTGCTTGTTGGACAAAATGACACAGTAACGGGGCACTGAATATTAACGTTATGATTAACCAGATCACCTGTCATTCAAACTACAGATGAAGGGTTAATAGTGACTAGATTTACCATGTCCACTTACTCTATTCTCACCTGCCTGCAAACAG
Seq C2 exon
GTCATTCCCCGCAGAGCTGAATGACATATTTTCCAGTTGGGTATCAGATTGTGAGGATCGAGGTCAACAGGACATTGGACAACGTCTTATATCAGCATCTCTGTTCCTTCGATTCTTATGCCCAGCGATCCTCAGTCCATCACTCTTTGGCCTTACCCAACCCTATCCAGAGACAAACACCCTTCGCACTCTTACTTTAACAGCCAAAGTCATTCAAAACCTTGCAAACTTCACCTT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000089933:ENSDART00000124643:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=FE(27.9=100)
A:
NA
C2:
PF0061614=RasGAP=PD(44.2=95.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAACTGTGAGGTAGACCCGCT
R:
AGGTGAAGTTTGCAAGGTTTTGA
Band lengths:
347-779
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]