DreINT0126419 @ danRer10
Intron Retention
Gene
ENSDARG00000005989 | rgl1
Description
ral guanine nucleotide dissociation stimulator-like 1 [Source:ZFIN;Acc:ZDB-GENE-040426-1816]
Coordinates
chr8:19265092-19265889:-
Coord C1 exon
chr8:19265674-19265889
Coord A exon
chr8:19265196-19265673
Coord C2 exon
chr8:19265092-19265195
Length
478 bp
Sequences
Splice sites
5' ss Seq
CAGGTGCAT
5' ss Score
4.89
3' ss Seq
TGTGGATGACGTGTTTTCAGGAG
3' ss Score
4.88
Exon sequences
Seq C1 exon
CTGTTATTTAAGAAGGTGGTTCCTCATCACTGTTTGGGCTCGATTTGGTCTCAGCGTGATAAAAAGGATGGCAAACAGAGTGCACCAACCATCCGTGCCACCATCACGCAGTTTAACGCGGTAACGGCGTGTGTGGTCAGCACTATCCTCCGTCAGCGGCAGATACGCCCGCATTTGCGTGCTCGCATCATACAGCGCTGGATCGACATCGCACAG
Seq A exon
GTGCATAAACCAACACACGAACACACACATTCTTTAATGGTCAAGCAGTCAAATTTGACTTTATGCTAACATTTGGCCTGCGTTTAAAGCTAACAAGCGTACACATTTCAGATTAAAATCTATCCATGTTGCATGATTTTCATGCAAAACACAAGATTAAGTTTCAAAGCCTATTTTATGTTTAAAATGTGTTTAAAGTGACACCCAATCCATGTCTTTGTGTGACATAACTATTTTAGATTACATCTAGATCATCCAAAGCCAAAAGATAAACCCAGATAAGCTGCAGATAAATCCTAATGTTGATCTGGAATTTTTACCAGCACTTAAATCCATGTCATTGTGTAACATAACTGTTTTAGATTAGATCTAGATAAGCCAAAGCAGACATAATAATGTTATTGAAACTCCAAAAGGTGTTCTGATAAAGCTTCATTAGGCCCATTTGTATGTGTAAATGTGGATGACGTGTTTTCAG
Seq C2 exon
GAGTGTCGCATTCGGAAGAACTTCTCGTCTCTAAGAGCCATCGTGTCTGCTCTGCAGTCCAACCCCCTCTACAGACTAAAGAGGACATGGGCATCTGTTGCTAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000005989:ENSDART00000138881:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.014 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=FE(32.0=100)
A:
NA
C2:
PF0061714=RasGEF=FE(15.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGTGGTTCCTCATCACTGTT
R:
CAACAGATGCCCATGTCCTCT
Band lengths:
303-781
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]