DreINT0150014 @ danRer10
Intron Retention
Gene
ENSDARG00000035468 | slc25a25b
Description
solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25b [Source:ZFIN;Acc:ZDB-GENE-060526-340]
Coordinates
chr5:31204716-31206144:+
Coord C1 exon
chr5:31204716-31204883
Coord A exon
chr5:31204884-31205993
Coord C2 exon
chr5:31205994-31206144
Length
1110 bp
Sequences
Splice sites
5' ss Seq
GAGGTGAGT
5' ss Score
10.03
3' ss Seq
GCTCCTATTTTTTCCTGTAGACA
3' ss Score
8.83
Exon sequences
Seq C1 exon
GTTTTAAAGACTCGTCTTGCTCTGGGAAGGACGGGTCAGTACTCGGGAATTGCAGACTGTGCTAAACATATTTTTAAGAAGGAGGGCATGACAGCTTTCTATAAGGGTTACATTCCCAATATGCTGGGTATCATCCCTTATGCTGGAATAGACCTGGCTGTTTATGAG
Seq A exon
GTGAGTTTTACACTGTAAGAAACGGAATATATACTTCTCAGGCTACATCGTTGTTGTTGTTAACTGAATATGAACATTTAATATTAAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATTTTATACAATATATAATTTTAATTATATCAATTATATTATGCCATCAAAACACACTCAAAAAAATTATTTAGTAGTTTTTCAAGCTGCTTATTTTAAATGAGCTGAAATAACAAGTTTTTTGAGAAATCTGTTTTTGGAACAACTCACTTGTTTTATGTTCAATCCACCTACATTTGTTACGTTAAATTAATTGATTTGGGCTGGGCAAACAGGAATGAATTATGTAAAACACTGCATCATTTTCTAGTAAATATATGTTTTAGTTCTAGTTTGTTTATTTATAAAGCACATTTAAAAACAGACACAAGAGTGACCAAAGTGCTGTACATAAGTTAAAGTAAAATAAAAAGAAGAATATAAAAACATGAAAATAAGTCAAGAGAAATGACTGAATAAATTTAAAAATGAAGCAGTAGAAAGAACTATTAAAATAATATTGAAAAACTAAGCTAAATAGGTACCTTTAAGGAGTAGTTTAAAAATCGATAGTTATGGAGCCATTCTAATCTCAAGGGGCAAAGTGTTTCATAACCGCAGACCCACTACAGAGACAGCTCTGTCCCCTCTACGTTTCAGTCTGGACTGAGGATATAAAAGTAGATTTTGATCACTTGATCTGAGAGATAGGTTGAGGCTAGATTATGAAGAGAGTTACATACCAGTAAAAGCATTTTAAAATCAATTCTGAATTGGATGGGCAGCCAATGGAGGCCCCCTTAAAAAAATAAAAAAAAATAAAAGCAATCCAAGAGAAAACGAAAGTCATAAGTATACAGGTATATAAATTTAATATAAATATAAATTTGTTTTTGTGTTTTACTTCAATCTTTATTTTAGTACAGTTGTGTTAATGGATAGTTAATAATAATTTAAATGGCATTGCTATTTTATATATTACACTGTGATTTAACTCAATATCGCTCCTATTTTTTCCTGTAG
Seq C2 exon
ACACTGAAGAACTCATGGCTGCAGAGGTTTGCCACAGACAGCGCTGATCCGGGTGTGTTTGTGCTGCTAGCTTGTGGTACAATGTCCAGCACATGTGGACAGTTAGCGAGCTACCCGTTAGCCTTAGTGAGGACACGCATGCAGGCGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035468:ENSDART00000098163:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0015322=Mito_carr=FE(59.1=100)
A:
NA
C2:
PF0015322=Mito_carr=PD(9.7=17.6),PF0015322=Mito_carr=PU(37.5=70.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGACTCGTCTTGCTCTGGGAA
R:
TGCATGCGTGTCCTCACTAAG
Band lengths:
304-1414
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]