DreINT0160570 @ danRer10
Intron Retention
Gene
ENSDARG00000003520 | tbc1d14
Description
TBC1 domain family, member 14 [Source:ZFIN;Acc:ZDB-GENE-030131-6136]
Coordinates
chr7:39422659-39423332:+
Coord C1 exon
chr7:39422659-39422776
Coord A exon
chr7:39422777-39423225
Coord C2 exon
chr7:39423226-39423332
Length
449 bp
Sequences
Splice sites
5' ss Seq
CATGTGAGT
5' ss Score
7.83
3' ss Seq
CCTTTTCCACACCATTCCAGGTG
3' ss Score
9.48
Exon sequences
Seq C1 exon
AGCTGAAAGATGCCCAGAAACGAAAGAAACAGCTGGAGGACCGGTGTAAACTAGAGGAAAGCATTGGCAATGCTGCTCTCACCTGGAGTCAGGAGATATTACCCAACTGGCAAAGCAT
Seq A exon
GTGAGTTAAAGTGGAGACTGTGTCAGAGATATTATCACATTTATAGTTAAAACGTTTGTCATTTATTGATGTTCCATGTAAAGAATAACCTCAATAGATTAGAGTAGCTGAAAAACTTTGTAAACAGAATAGTTGACAAGTGATGGACGGATTGATGTAAACTTAGTGAGAGCCACGAATGTCTCTGTTGTGTGCTGAATTGAGTTTAAATCTGGCTTGCAACATTTTAAGGTCCAGTTTAACAATGTACCATTTTTCTGCTTTGTTATATCCTATAATCTCTCTTTTGTCCCAATTATACAAAATATAAAAGTAGATATAAAAAAGAATGTAAAAATATGGTTTGAGAAATAGACAGTATATTCATGCATACTGAAGTGAAATTACTTCACTCTGCTAAGCTTAAACTCACTTGGATTTTGACTTTGTCCTTTTCCACACCATTCCAG
Seq C2 exon
GTGTTCATCGAAGCGGGTGAGGGATTTATGGTGGCAGGGTCTGCCACCCAGTGTGAGGGGTAAAGTCTGGAGTCTGGCAATAGGAAATGAATTGAACATCACAGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000003520:ENSDART00000133420:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.375 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF085986=Sds3=FE(20.4=100)
A:
NA
C2:
PF085986=Sds3=FE(18.8=100),PF0056613=RabGAP-TBC=PU(8.7=54.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGAAAGATGCCCAGAAACG
R:
CATCTGTGATGTTCAATTCATTTCCT
Band lengths:
224-673
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]