DreINT0170298 @ danRer10
Intron Retention
Gene
ENSDARG00000037559 | uba1
Description
ubiquitin-like modifier activating enzyme 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2009]
Coordinates
chr23:20729465-20729907:+
Coord C1 exon
chr23:20729465-20729630
Coord A exon
chr23:20729631-20729710
Coord C2 exon
chr23:20729711-20729907
Length
80 bp
Sequences
Splice sites
5' ss Seq
CCCGTAAGT
5' ss Score
9.09
3' ss Seq
ATCATGTTTGCTCCTTCCAGGTA
3' ss Score
9.5
Exon sequences
Seq C1 exon
AAGATGAAGAGTGAGACTGCTGCTGCGGCAGTGAAGCAGATGAACCCGTCAGTCCGTATCACTGGTCACCAGAACCGTGTGGGACCGGACACGGAGAAGGTCTATGATGATGACTTTTTTGAATGCCTTGATGGAGTGGCAAATGCTCTTGACAATGTAGATGCCC
Seq A exon
GTAAGTAAGAGTTTTAAGAAATCGTGCAGCTGCTCACAGTTGAAAATGCATTGCTTGAACATCATGTTTGCTCCTTCCAG
Seq C2 exon
GTATGTATATGGATCGGAGGTGTGTGTACTACCGTAAGCCATTGCTGGAGTCTGGTACTCTTGGCACCAAAGGAAACGTGCAGGTGGTCATTCCCTTCATCACAGAGTCCTACAGCTCCAGCCAGGATCCTCCAGAGAAGTCCATTCCTATCTGCACACTCAAGAACTTCCCAAATGCAATCGAACACACCCTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000037559:ENSDART00000054691:15
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.134 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0089916=ThiF=FE(38.2=100)
A:
NA
C2:
PF0089916=ThiF=PD(20.8=45.5),PF105854=UBA_e1_thiolCys=PU(73.3=50.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACCCGTCAGTCCGTATCACT
R:
GTACCAGACTCCAGCAATGGC
Band lengths:
182-262
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]