DreINT0170302 @ danRer10
Intron Retention
Gene
ENSDARG00000037559 | uba1
Description
ubiquitin-like modifier activating enzyme 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2009]
Coordinates
chr23:20732470-20732827:+
Coord C1 exon
chr23:20732470-20732544
Coord A exon
chr23:20732545-20732637
Coord C2 exon
chr23:20732638-20732827
Length
93 bp
Sequences
Splice sites
5' ss Seq
AATGTGAGT
5' ss Score
7.8
3' ss Seq
ATCTGATGTGTGGTTTCCAGGAT
3' ss Score
8.63
Exon sequences
Seq C1 exon
CTTACAAGCTCTGGGGCGCCATTCTGGTCAGGTCCAAAGAGATGTCCTCATCCATTAGAATTCAGCACTAACAAT
Seq A exon
GTGAGTACTTGCCCTAAACAACTGAAAGAAACATTTGTCTAATTTTACTGGGTAAAAACTGATTGATTCTGATATCTGATGTGTGGTTTCCAG
Seq C2 exon
GATCTTCACATGGACTACATTTTGGCAGCAGCTAACTTGTATGCCCTGTCATACGGCCTGCCATCCTGCAATGACCGCTCAGCTCTGACCAAGCTTTTGCAGGATATTAAAGTGCCTGAGTTCACACCCAAGTCAGGAGTCAAGATTCACGTGTCTGATCAGGAGCTGCAGAGTGCCAACGCATCAGTCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000037559:ENSDART00000054691:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.120 A=NA C2=0.047
Domain overlap (PFAM):
C1:
PF0213416=UBACT=PU(7.4=20.0)
A:
NA
C2:
PF0213416=UBACT=PD(89.7=95.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTCAGGTCCAAAGAGATGTCC
R:
GGGTGTGAACTCAGGCACTTT
Band lengths:
179-272
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]