DreINT0173882 @ danRer10
Intron Retention
Gene
ENSDARG00000079564 | vmhc
Description
ventricular myosin heavy chain [Source:ZFIN;Acc:ZDB-GENE-991123-5]
Coordinates
chr2:24607417-24608099:-
Coord C1 exon
chr2:24607857-24608099
Coord A exon
chr2:24607594-24607856
Coord C2 exon
chr2:24607417-24607593
Length
263 bp
Sequences
Splice sites
5' ss Seq
AAGGTCTGG
5' ss Score
3.93
3' ss Seq
GTATGCATGTTCTTTTACAGGTA
3' ss Score
10.71
Exon sequences
Seq C1 exon
GAGCAAGACAATCTCTGTGATGCAGAGGAACGATGTGACCAGCTCATCAAAAACAAGATTCAGCTTGAGGCTAAAGCCAAAGAGCTCACCGAGCGACTTGAGGATGAGGAGGAGATGAATGCAGAGCTGACAGCTAAGAAGAGAAAGCTGGAGGACGAATGCTCTGAGCTGAAGAAGGATATTGATGATCTGGAGCTCACTCTGGCTAAAGTCGAGAAAGAGAAGCATGCTACTGAGAACAAG
Seq A exon
GTCTGGTTTGCCTTTTTATACAAGCTCTTAGATGTACAAATTATTGCAAATTTAGCCCATCACATGGCATTGTTTTGGATGTATGTTAAGTTCTGTGCTATAAACGGATTTCTTTATCTTCAAATTTGGTCTTAAAGATGTCAGTGTGAATGCTAAATGAATGGGGACTAAATGTAATATTTCCCTTTTTTGTCTGGACCAAACAAACAAGAGATCAACCGTACAAGTGTAAAAACATCCTTAGTATGCATGTTCTTTTACAG
Seq C2 exon
GTAAAGAACCTGACAGAAGAAATGGCAGCTTTGGACGACATAATCGCAAAGCTGACCAAAGAGAAGAAAGCCTTGCAAGAAGCTCATCAGCAGACACTGGATGACCTGCAGAGTGAGGAGGACAAAGTCAACACCCTCACCAAGGCAAAAGCAAAGCTAGAGCAACAAGTAGATGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079564:ENSDART00000099532:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.407 A=NA C2=0.559
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(41.9=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=PD(28.8=93.2),PF0157614=Myosin_tail_1=PU(43.1=47.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGACTTGAGGATGAGGAGGA
R:
CTTTTGCCTTGGTGAGGGTGT
Band lengths:
302-565
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]