DreINT0173888 @ danRer10
Intron Retention
Gene
ENSDARG00000079564 | vmhc
Description
ventricular myosin heavy chain [Source:ZFIN;Acc:ZDB-GENE-991123-5]
Coordinates
chr2:24605702-24606099:-
Coord C1 exon
chr2:24605981-24606099
Coord A exon
chr2:24605899-24605980
Coord C2 exon
chr2:24605702-24605898
Length
82 bp
Sequences
Splice sites
5' ss Seq
AAGGTGTGA
5' ss Score
4.59
3' ss Seq
AATTATGCAACCTCCTCCAGGCA
3' ss Score
6.05
Exon sequences
Seq C1 exon
GTGAACTTTCAAGACAGCTGGAGGAAAAGGACTCTCTTGTCTCCCAGCTAACCAGAAGCAAGATGTCTTACACTCAGCAAATTGAAGATCTTAAAAGACAACTGGAGGAGGAAACAAAG
Seq A exon
GTGTGAATTGAGTTTAAAATTCTTTTTTAATTATTTGCCATCATTTTTTTGGTCTGTTAAATAATTATGCAACCTCCTCCAG
Seq C2 exon
GCAAAAAGCGCTCTCGCCCATGCTGTACAGTCAGCCCGTCATGACACAGATCTGCTTAGAGAGCAGTATGAGGAGGAGCAGGAAGCTAAAGCAGAGCTACAGCGAGGCATGTCCAAAGCTAATTCTGAGGTGGCACAGTGGAGAACCAAGTACGAAACTGATGCCATCCAGAGAACTGAAGAACTGGAGGAAGCCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079564:ENSDART00000099532:28
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.850 A=NA C2=0.879
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(4.5=100),PF0003816=Filament=FE(34.2=100),PF059116=DUF869=FE(38.6=100),PF0003816=Filament=FE(11.7=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.6=100),PF0003816=Filament=PD(24.6=42.4),PF059116=DUF869=PD(20.8=31.8),PF0003816=Filament=FE(19.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGACTCTCTTGTCTCCCAGCT
R:
TCCTGCTCCTCCTCATACTGC
Band lengths:
174-256
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]