DreINT0174580 @ danRer10
Intron Retention
Gene
ENSDARG00000075441 | vwa2
Description
von Willebrand factor A domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-100302-1]
Coordinates
chr12:30202029-30204541:-
Coord C1 exon
chr12:30204434-30204541
Coord A exon
chr12:30202608-30204433
Coord C2 exon
chr12:30202029-30202607
Length
1826 bp
Sequences
Splice sites
5' ss Seq
GTGGTAAGT
5' ss Score
10.36
3' ss Seq
GTATTGATTTTCATTTCCAGCTC
3' ss Score
9.43
Exon sequences
Seq C1 exon
ACCCCTGTGACTCTCAGCCCTGTCAGAATGGGGGGACGTGTGTTTCTGAAGGACTGGATAAATACCGGTGCGAGTGTCCTGCTGGTTACGCCAATGACCCCAATTGTG
Seq A exon
GTAAGTTCACATTCATATGCTTCTTTTCCCACAGTTCTTCTCATGTGGTGAGCTGCCCCTCTCTCACATTCACGCTTAGATTATTTTGTTTTGGTGGGTTTGGCTGGATCAGATGTGTGTGTGTGTGTGTGTGTGTGGGTGTGGGTGTGGGTGTGTGGGTGTTGTCACGGGTGTTCAAGTTACAGTGGGGATCGGTTTTCAGGCAGGGTCATGGTTGACACTCTAATTAGCTAAAGGCAATCCAAAAGTTTCCAAAGTCAGCAGTTTCTTGTAAATGTGCAGCTTTGTTCTGTGAAATTAAAATTATTGATAAAAATATTTTATAAATATTTTACTGATTAAAAAACATACAGTATTGCAGAATTATAATATAATATAATATAATATAATATAATATAATATAATATAATATAATATAATATAATATAATATAATATAATATAATATAATATAATATAATAACACTGTCGCCTCACAGCAAGAAGTTGGCTGGTTTGAGTCCCAGCTGGGCCAGTTGCCATTTCTGTGTGGAGTTTTTCCCGGTTTCCCCCACAGTCCAAAAACATGTGCAATAGGTGAATTGGATGAACTAAATTGGCCCTAGTGTATGAGTGTGTGAATGTGAGAGTGTATGGGCGTTTCCTAGTACTGGGTTGCAGCCAGAAAAGCATCCGCTGCTTAAAACAGATGCTGGAATAGTTGGTGGTTCATTTCACTGTGGCAAACCCCTGACAAATAAGGCACATAGCCGAAGGAAAATTAATATAATATAATATAATATAATATAATATAATATAATATAATAAATGGGGTGGATGAGGAGATTCCCCCCAAAAATGTGTGTAAATTGAGTGTCCAGAAAAAATTATTAATTATGAACAACAGAAAGTAATAAAATATAAATATTCTTACAAAAAAAATATTCTTACAGCAATTTCTGTTTTGGCCCATAATACAGTATGTGGTCCTTGTGTCTACGTTTGTTTTGTTTTTGTTTAGGTTCTGCAGTAGCTGCACCGAAAATAACTGTATAAAAGAAATTAGCAATAGGTAAACCCCAAATGCACATGCTACAATACTTTTATAATGGGCCTTGTCATTTGCTAAATTACACTGTGAGACAGGAGCCATCATCGGTCCCCATGAGTTCTATTGAAATGCGGACACTAAATCCTACATCCAATCAGAACGTGATGACATACCTAACCTCCTGCTGTCAATCAATGTGAATGACATCACAAGTAGAGGTTAGATCAGATGACCCCACTTACCTGCAGAGGGATATGTGGAGCTTTGTGTTTGTTTAGGCTGGTTGTAAAGGCATTTTAGCAAGTGTTAATGTGTAAAGGAGACTAAAGCTCCTATGCTTTTCTCCTCCTTTAACAATGTAATGTCTCATATGATTCTAACTAAAATCTAAAACAAACAGGATATATGTAATGCAGACTAACAGGTATGTAACCAAAGCTGGAAACCTGTAACCATTGGTTTCCATAGAATTTTTTTTTATTATGGAAGTTAATGGGTACAGGTCATCAGCTTTCTTCAAAACATCTTCATTTGAGTAAATAGTGAGTAAATTTTCATTTTGAGTAAACTATCCCTTTAAGGATAGTTAAAGGTTTAGTTCTACCAAAAATGACAATTTGCTGTTTTTTTTCACCTTCAGCCCATCCAAGTGGTACATATTTACATGAACACATGTTTTTGTCTTCAATAGAACATTAAAGAACTTTTTAGCTGAAATTATTGTACTTGTTGATAAAAACTGAGGCAAAAATGTCTCTAATGTCCTCATATTTAATTGTATTGATTTTCATTTCCAG
Seq C2 exon
CTCCCATTCTGAGTTTTGACTGCTCCGTGGATGTGCTCTTCCTGCTGGAGGGCTCCTCTGCTCTTACTCTGGAGGGTTTCTTGCGATTCAAGTCCTTCCTGAAGCGTTTCATGCAGGCCGTATTGAGCTCTGACACTCCTGTAAAAATGGGGTTGGCCCAGTATGGCAGCGACGTGAAGATCGAGGCCAGAATTGGGGAGCACAGAGACCCGGTGAAGCTGATCCAGGCTGTTGAGGGACTTCAGTATCGTGGTGGGCAGACGAAAACCGGAAATGCCCTTCGCTACATCACACGCCACGGCTTCCAGAGCACGCCTGTGTTCGCTGACGTTCAGGACGACCTGCCGCGGGTGGTAGTTCTGCTCACCGGGACGCCCTCAGCAGATCATGTGGTGGAGCCAGCGAAGTACGCACGGGACAGGGAGATCTTCATCATCGGTGTAGCGCCAGAAGGCATGAGGGCTGAGATAAACAACATCACAGGGAACCCTCAGCGCACCATCATGTACCAGTCACCGGATAGATTGAGCAGCAAGATCCCAGAACTAAGAGCCAAAATCTGCAGCGTCGACAACCAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075441:ENSDART00000152985:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.052
Domain overlap (PFAM):
C1:
PF0000822=EGF=WD(100=83.8)
A:
NA
C2:
PF0009223=VWA=WD(100=89.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGGACGTGTGTTTCTGAAGG
R:
GCATTTCCGGTTTTCGTCTGC
Band lengths:
354-2180
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]