DreINT0174718 @ danRer10
Intron Retention
Gene
ENSDARG00000077231 | vwf
Description
von Willebrand factor [Source:ZFIN;Acc:ZDB-GENE-070103-1]
Coordinates
chr18:7205098-7205493:+
Coord C1 exon
chr18:7205098-7205144
Coord A exon
chr18:7205145-7205356
Coord C2 exon
chr18:7205357-7205493
Length
212 bp
Sequences
Splice sites
5' ss Seq
CTGGTAAGC
5' ss Score
8.69
3' ss Seq
TGTCCTCTTTCTATCTATAGGTG
3' ss Score
13.16
Exon sequences
Seq C1 exon
CGTCTGTCGGCATGGATCATGGGAATGCACTAATGAAGGATGTCCTG
Seq A exon
GTAAGCAAATATTTACAGTTGAAGCCAAAATCATTAGCTCTCCAGTGAAATCTATTATATTTATTAGGCTCTTCACGTTTGAGTTGAGATTACGTTAAACTTTGAATTGAAAATTGACATTTCTAACAACTTCATGGGAACTTTGGGGGGGGGGGGGGAGTAATATTTTAAATAAAAAATTCTGATTCATTTTGTCCTCTTTCTATCTATAG
Seq C2 exon
GTGAGTGTTTGGTGACAGGACAGTCGCATTATAAGACCTTTGACAACAAATTCTTCACCTTCAGTGGGATTTGTCACTATCTGCTTGCTAAAGACTGCCAGAGCAACTCTTTTTCTGCCGTCATTGAGACTGCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000077231:ENSDART00000134872:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0009313=VWC=FE(30.2=100),PF053758=Pacifastin_I=FE(50.0=100)
A:
NA
C2:
PF0009313=VWC=PD(30.2=34.8),PF053758=Pacifastin_I=PD(9.4=6.5),PF0009420=VWD=PU(27.9=93.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGTCTGTCGGCATGGATCATG
R:
AATGACGGCAGAAAAAGAGTTGC
Band lengths:
172-384
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]