GgaINT0134963 @ galGal4
Intron Retention
Gene
ENSGALG00000001040 | MEGF6
Description
multiple EGF-like-domains 6 [Source:HGNC Symbol;Acc:HGNC:3232]
Coordinates
chr21:1017560-1017915:+
Coord C1 exon
chr21:1017560-1017691
Coord A exon
chr21:1017692-1017786
Coord C2 exon
chr21:1017787-1017915
Length
95 bp
Sequences
Splice sites
5' ss Seq
AGAGTGAGT
5' ss Score
7.36
3' ss Seq
CTGTGCCCTCTGTCCCACAGCCT
3' ss Score
10.36
Exon sequences
Seq C1 exon
TCTGTCTGGAGGACACCTTTGGCCCCGACTGCAGCCTGACATGTGAGGACTGTCAGAACGGGGGCACATGCAATGTGGAGGGGACGGGCTGTGAGTGCCCGGCCGGCTGGAGCGGGCTGCTGTGCAACCAGA
Seq A exon
GTGAGTGATGCTGCACACAGACCCTCATGCAATCTTATTCAGAGCTCAAACCCACAGATGTGCATGCTGTTAAAGCTGTGCCCTCTGTCCCACAG
Seq C2 exon
CCTGCCCGCCTGGAACCTTCGGCAAGAACTGCAGCCAGCTCTGCCTCTGCCAGAATGGGGGTACCTGTGAGCCCGCCACCGGCACCTGCCGCTGCCCCCCTGGCGTGGCTGGGATCCACTGTGAGGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000001040:ENSGALT00000001544:15
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF079748=EGF_2=WD(100=75.6)
A:
NA
C2:
PF0005319=Laminin_EGF=PU(63.0=65.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCCTGACATGTGAGGACTGT
R:
TCACAGGTACCCCCATTCTGG
Band lengths:
170-265
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]