MmuINT0097659 @ mm10
Intron Retention
Gene
ENSMUSG00000057751 | Megf6
Description
multiple EGF-like-domains 6 [Source:MGI Symbol;Acc:MGI:1919351]
Coordinates
chr4:154254542-154256124:+
Coord C1 exon
chr4:154254542-154254673
Coord A exon
chr4:154254674-154255995
Coord C2 exon
chr4:154255996-154256124
Length
1322 bp
Sequences
Splice sites
5' ss Seq
AGAGTGAGG
5' ss Score
2.6
3' ss Seq
CTCCCTGTCATCCCCTGTAGCTT
3' ss Score
9.89
Exon sequences
Seq C1 exon
TCTGCTTGGATCACTCCTTCGGCCATGACTGCAGCCTAACCTGCGATGACTGCAGGAATGGGGGGACCTGCTTCCCGGGCCTGGATGGCTGTGACTGCCCAGAGGGCTGGACCGGGATCATCTGCAATGAGA
Seq A exon
GTGAGGCTGCTGGTGGGGTCTAGGCAGAGTCTGTGAACCCCAAGAGCTGTGATTCATTCTGTATGGTTCCCCCTGCCTAGCTCTGGGGAGAAGAATCTGAGTATGTCCACACACCCCTCTGTCTGACCATCTGTCCACTCACCCATCTGTCTATCCATCTATCAATCTGCCCTTTTACCTACCCACCTACCTACCCATCCAACTGTCTACATATCCATCCATCTGTCTACTCACCTACCCATCCATCCATCTACACACCTATCTATCCGTCTACACACCCATCCATCCATCTATACACCCATGTACCCATCTATCCACTTGTCTACCTACCCACCCATCCATACATCTATCTCTACAATCATGTACCCATCCATCCATCTATATACCTATGTACCCATCCATCCATTTATCCACCCATCCATCCATCTACCCACCCATCCATCTATCTGTCTACCCACCCATCCATCGATCTGTCTACCCACCCACCCATCCATCCATCTACCCACCCATCCATCTGTCTACCCACCCATCCATCGATCTGTCTATCCACCCACCCATCCATCTATCTCCTTACCCACCCAGCCATCCACCCACCTATCCATTCACCTATCCATCCATCCATCCATCCATCCATCCATCCATCCATCCATCTATACACCCATATACCCATCCATCTATTTCCATACTTACCCACCCATGCATCCTTCTCTACATCCATGTACCCATCCATTCCATTAATCCCACCTATTCACCATTTTATCGGTTCATCCCTCCCTTTGATAAGTGTCTGCCAACTGTTCCCCAATCCCTGCCCCTTGTAATAGACATGGTGCTAGGATGTGGAGCACTCTGGAAAGCATCAGTCTCTAGGCATCTAATGACAAATCAACAAATAGCTAAGACTGTTGGAGGTGTCTGTGGAGGAGAGCTAGTGGGCGGGCTTCTCTTGTACAGGTAGTGGGGATTCTGAACCATCTGATGGGAGCAGAGCCTTGCTTAGGACTACATAACAAATTAGTACCTGAAGCACCCTGAAAATGGATCTATCTCCAGTCTTCCTTATCCTTCCCAAAGCCACCAGGCCCTTCAGGCTGTGAGGATCACCTGAAATAGGTCATCTGATTAAATGGGTCCTTGCGTGTGTTAGATGTCATGATGTAGCCATTGATTAGTGATGTCTGCCCTGAGCATGGTTGGGGCCTAGAGGCACAGATGGCATCATTTCCTGTTTCAGGCTGTTGTTTAAAATGGTTGCCTTTAATGCCAGGAGAACCTGAGTTCGGCTGTTGGTAATCCTGGTGTCTCCCTGTCATCCCCTGTAG
Seq C2 exon
CTTGTCCTCCAGACACTTTTGGGAAGAACTGTAGCTCTCCCTGCATCTGTCAGAATGGTGGGACCTGTGACCCGGTGTCAGGAGCCTGCCGCTGTCCCCCAGGCGTCAGTGGAGCCCACTGTGAGGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057751:ENSMUST00000030897:13
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0005319=Laminin_EGF=PU(63.0=65.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCTTGGATCACTCCTTCGG
R:
CATCCTCACAGTGGGCTCCA
Band lengths:
260-1582
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types