HsaEX0027617 @ hg19
Exon Skipping
Gene
ENSG00000250571 | GLI4
Description
GLI family zinc finger 4 [Source:HGNC Symbol;Acc:4320]
Coordinates
chr8:144349607-144356972:+
Coord C1 exon
chr8:144349607-144349654
Coord A exon
chr8:144351530-144351690
Coord C2 exon
chr8:144356874-144356972
Length
161 bp
Sequences
Splice sites
3' ss Seq
ATGGTTTTCATTTTCCCCAGGTC
3' ss Score
10.46
5' ss Seq
ATGGTACTC
5' ss Score
3.28
Exon sequences
Seq C1 exon
GGCCGGACACTTCCGTCCGGCGCGCGGCGTCCTCCTCCCGCTCGGAAG
Seq A exon
GTCCCAGGTGTGACACCTTCAGCAGGTCTCAGGGAAGATGGCAGCCCTAGGGGACATTCAGGAGTCCCCTTCTGTCCCGTCCCCTGTCAGTCTCTCATCACCAGGGACACCTGGAACCCAGCACCACGAGCCTCAGCTTCACCTCCATGGGCATCAACATG
Seq C2 exon
GCTCCCCTGGCTCCAGCCCTAAGGTGCTCTCCCAGCCGTCCGACCTGGATCTCCAAGACGTAGAGGAAGTGGAGATCGGCAGAGACACCTTCTGGCCCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSG00000250571_MULTIEX1-1/4=C1-3
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref, Alt. ATG (>10 exons))
No structure available
Features
Disorder rate (Iupred):
C1=NA A=1.000 C2=0.902
Domain overlap (PFAM):
C1:
NA
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCTCCTCCCGCTCGGAA
R:
CCAGAAGGTGTCTCTGCCGAT
Band lengths:
114-275
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
This event
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Genotype-Tissue Expression Project (GTEx)
- Autistic and control brains
- Pre-implantation embryo development
Other AS DBs:
FasterDB (Includes CLIP-seq data)
AS-ALPS (AS-induced ALteration of Protein Structure, links to PINs)
APPRIS (Selection of principal isoform)
DEU primates (Only for human)