HsaEX6016764 @ hg19
Exon Skipping
Gene
ENSG00000091428 | RAPGEF4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:16626]
Coordinates
chr2:173831989-173850221:+
Coord C1 exon
chr2:173831989-173832172
Coord A exon
chr2:173848224-173848308
Coord C2 exon
chr2:173850161-173850221
Length
85 bp
Sequences
Splice sites
3' ss Seq
TTTTTTTTTTATCTTTGTAGACC
3' ss Score
10.79
5' ss Seq
ACAGTAAGT
5' ss Score
9.49
Exon sequences
Seq C1 exon
TGGACCAGGAGCACCATTTCCAAGACAAATATTTATTCTATCGATTTCTGGATGATGAGCACGAGGATGCCCCTTTGCCTACTGAGGAGGAGAAGAAGGAGTGTGATGAGGAGCTCCAGGACACCATGCTGCTGCTGTCACAGATGGGCCCCGACGCCCACATGAGGATGATCCTTCGCAAACC
Seq A exon
ACCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTATGAGGAGCTTCTTCATATTAAAGCCTTATCCCATCTTTCTACCACA
Seq C2 exon
GTGAAACGAGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGGACTGTGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSG00000091428-'20-22,'20-21,21-22=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.226 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061016=DEP=PD(21.1=24.2)
A:
NO
C2:
PF0002724=cNMP_binding=PU(10.3=42.9)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGCCCCTTTGCCTACTGAG
R:
CTCCTTTGGCGTGAGACTCAA
Band lengths:
171-256
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Autistic and control brains
Other AS DBs:
FasterDB (Includes CLIP-seq data)
AS-ALPS (AS-induced ALteration of Protein Structure, links to PINs)
APPRIS (Selection of principal isoform)
DEU primates (Only for human)