MmuEX0004114 @ mm9
Exon Skipping
Gene
ENSMUSG00000030609 | Aen
Description
apoptosis enhancing nuclease [Source:MGI Symbol;Acc:MGI:1915298]
Coordinates
chr7:86040740-86050929:+
Coord C1 exon
chr7:86040740-86040900
Coord A exon
chr7:86047230-86047716
Coord C2 exon
chr7:86050729-86050929
Length
487 bp
Sequences
Splice sites
3' ss Seq
CAGTGTCCATCTCTCACCAGCCT
3' ss Score
5.85
5' ss Seq
GAGGTAAGG
5' ss Score
10.28
Exon sequences
Seq C1 exon
GAGGAGGAGCAGGATCAACCAATCAGAAGAGTCCTTAGGCGGGCGCTACGCCCCAGAGAACCAATAGACAGGTAGCCGAAGCCGGGCTTGTCCGGGCAAGGAGCTTCCGCACCCGGCAAGGGTTCCACGTGAAACCTGGGAAAGCGCGGCCGGGTTTTCAG
Seq A exon
CCTGAATACCAAGGATGTGGTTCGGAGAAGACACAAGAGGAGAAGCCGGCAGCACCAGCGATTCATGGCCCGCAAGGCCTTGCTTCAGGAGCAGGAGCTGTTGAGCACGGCACCAGGGCCAGGTCTCTGCCTTCTGCCTTCACCATCACAGATGCCAGCGGTCACAGAGGCTTCGGACAGCAGGAGGCAACGTCCCAAGGCCAGGTCCGGCAGCAATGGCCTGTGCAGCAAAAAGTCTGTCCCCAGAGAAGCTCCAAGACCTGGGCCCATCAAGTGTGTGGCCATTGACTGTGAGATGGTGGGTACGGGCCCCCAAGGACGGGTGAGCGAGCTAGCCCGCTGCTCTGTGGTGAGCTACAGTGGTGACGTTCTCTATGACAAATACATCCGGCCTGAAATGCCCATTGTGGACTATCGCACCCGCTGGAGTGGCATCACCCGCCAGCACATGCACAAGGCTATCCCCTTTCAGGTGGCCCAGAAGGAG
Seq C2 exon
ATCCTTAAGCTGTTGAAGGGCAAGGTGGTGGTGGGGCACGCACTGCACAATGACTTCCAGGCCCTGAAGTACGTCCACCCTCGGAGCCAGACCCGGGATACCACTTATGTCCCAAACCTGCTCAGCCAGCCCAGCTCCCTTATCCGAACTCGGGTCTCTCTTAAGGACCTGGCCTTGAACCTGCTGCACAAGAAGATCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030609-'0-4,'0-3,7-4
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion (Ref, Alt. ATG (<=10 exons))
Show structural model
Features
Disorder rate (Iupred):
C1=0.545 A=0.533 C2=0.063
Domain overlap (PFAM):
C1:
NA
A:
PF0092919=RNase_T=PU(44.2=48.9)
C2:
PF0092919=RNase_T=FE(42.3=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGAGGAGCAGGATCAACCAA
R:
TCTTGTGCAGCAGGTTCAAGG
Band lengths:
353-840
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types