Special

MmuEX0011719 @ mm9

Exon Skipping

Gene
ENSMUSG00000068917 | Clk2
Description
CDC-like kinase 2 [Source:MGI Symbol;Acc:MGI:1098669]
Coordinates
chr3:88974215-88978213:+
Coord C1 exon
chr3:88974215-88974331
Coord A exon
chr3:88977299-88977465
Coord C2 exon
chr3:88978119-88978213
Length
167 bp
Sequences
Splice sites
3' ss Seq
CTTCCCCTGTCTCTCCCCAGCCT
3' ss Score
11.42
5' ss Seq
AGTGTGAGT
5' ss Score
6.96
Exon sequences
Seq C1 exon
GGGCGGAACACAAGTTGCCCTGAAGATCATTAAGAATGTGGAGAAGTACAAGGAAGCAGCCCGACTAGAAATCAACGTGCTGGAGAAAATCAATGAGAAAGATCCTGACAACAAGAA
Seq A exon
CCTCTGTGTCCAGATGTTTGACTGGTTTGACTACCATGGCCACATGTGTATCTCCTTTGAGCTTCTGGGCCTTAGCACCTTCGATTTCCTCAAAGACAACAACTACCTGCCCTACCCCATCCACCAAGTGCGCCACATGGCCTTCCAGCTCTGCCAGGCCGTCAAGT
Seq C2 exon
TCCTCCATGATAACAAGTTGACACATACGGACCTCAAACCTGAAAATATTCTGTTTGTGAATTCAGACTACGAACTCACCTACAACCTAGAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000068917_MULTIEX1-1/2=C1-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0006920=Pkinase=FE(12.3=100)

							
A:
PF0006920=Pkinase=FE(17.7=100)

							
C2:
PF0006920=Pkinase=FE(9.8=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000113861





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000088445, ENSMUSP00000113390, ENSMUSP00000122634
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
No conservation detected
Human
(hg19)
chr1:155236520-155236686 
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
chr3:15427935-15428101 
HIGH PSI
BtaEX6014595
(CLK2)
Chicken
(galGal4)
JH375217.1:6664-6830 
HIGH PSI
GgaEX1015762
(CLK2)
Chicken
(galGal3)
chr25:1649039-1649205 
HIGH PSI
GgaEX0021338
(Q5ZJ82_CHICK)
Zebrafish
(danRer10)
chr19:9242312-9242478 
HIGH PSI
DreEX6072118
(clk2a)
Fruitfly
(dm6)
chr3R:28920537-28920627 
HIGH PSI
DmeEX6023982
(Doa)




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GGGCGGAACACAAGTTGCC

				
R: 
TCTCTAGGTTGTAGGTGAGTTCGT

				
Band lengths: 
210-377

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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