MmuEX0027477 @ mm10
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123461085-123480322:-
Coord C1 exon
chr4:123480187-123480322
Coord A exon
chr4:123465356-123465505
Coord C2 exon
chr4:123461085-123461234
Length
150 bp
Sequences
Splice sites
3' ss Seq
ACTTGCTGTTTATTTCCTAGGCC
3' ss Score
10.37
5' ss Seq
AAGGTAAGC
5' ss Score
10.22
Exon sequences
Seq C1 exon
GCTTTCAGAAGGAGAGAAGGAACAGATCTCTGAGCAGTTGCGTGTGCTGAACAAGACTTACCATGACCTTTGTGATGGTTCTGCAAACCAACTCCAGCAGCTCCAAAGCGAGTTGGCTCAGCAGACGGAACAAAAG
Seq A exon
GCCACAGTGTTAAATCGGCACAAGGAACTGCAAGGCAAACTTCAAGATCTGAGAGCCTGGGTCGGCAGAGCCAGTCTTACTCTGAACAGCAAGGGCTGTGACACTGAAACTGACGCTGACAGCCTGAGTCACACTCTCCAACCATACAAG
Seq C2 exon
ACTCTGCAGAAACAACAAGACACCTGCCATAAGAAGCTGGAGGATCTTTGCAACTGGGTGGGACAGGCAGAAAGAGCACTGGAGAGGCACCAGGGCGGAGCCTCTCGGCAAGAGCTCCCTGCTCTGCAGCAGAACCAAAGTGACTTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'72-75,'72-72,77-75
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.222 A=0.380 C2=0.484
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTTGCGTGTGCTGAACAAGA
R:
ACTTTGGTTCTGCTGCAGAGC
Band lengths:
242-392
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types