MmuEX0027474 @ mm10
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123461085-123480322:-
Coord C1 exon
chr4:123480187-123480322
Coord A exon
chr4:123471007-123476337
Coord C2 exon
chr4:123461085-123461234
Length
5331 bp
Sequences
Splice sites
3' ss Seq
GTTTTAATTCTTTCTTTTAGGGT
3' ss Score
11.63
5' ss Seq
CAGGCAAGT
5' ss Score
3.1
Exon sequences
Seq C1 exon
GCTTTCAGAAGGAGAGAAGGAACAGATCTCTGAGCAGTTGCGTGTGCTGAACAAGACTTACCATGACCTTTGTGATGGTTCTGCAAACCAACTCCAGCAGCTCCAAAGCGAGTTGGCTCAGCAGACGGAACAAAAG
Seq A exon
GGTTGCAGAGCAGTTGCTGGGGTGATTGACCTAGGCACAGTGGAGATATTCCCCATCTTCAGAGCCATGCAAAAGGGCCTCATTGACCAAGACACAGGCCTTGTGCTCCTGGAATCCCAGATTATCATGTCTGGCCTCATTGATCCCGAGAACAGTGAAAAGCTCTCCTTGGAAGAGGGTCTCACCAGAAATTTCATTAACCTTCCAATATATCAACAGCTCTTGGGACTAAGGGATTCTCTGTCTTTAGTAAGCAGGCTTACAGGGACCCTAGGCTCCCTTTCTGTGGTGGAAGCAATTGAAAAGAAAATAATCAGTGAGAGACTTGGACTGAAAGTCTTAGAAGTTCACCTGGCAACTGGAGGTTTCAGTCTCCCCCCTAGTGAGAACTGTATTAATCTGGAAGAAGCTTTTCATCAAGGCTTCATTGCATCAAGTCTTCACTCAGAATTACAGTCTCACCTGAGATCATCCAAGAATTTAATAGACCCCAATACAGCTGAAAAAGTTGGCTTGCTGGACCTGATGCAGAGATGTATCATCCATCAAGAGTCAGGGTTGAAATTGTTGCCTGTCAAGCAGTTGGCAGGGGGAATGGTGAGCTTGAAGTCAGGTCGGAAAGTTAGCATTTTCCGTGCTGTTCAGGAAGGGCTGATAGACAGGCAAGTCACTGTCCGGCTGCTGGAAGCCCAGCTATTTGCTGGTGGCATAGTAGATCCAAGAACAGGACACAGACTTACAGTGGAAGAGGCTGTGAGACATAATTTGATTGACCAAGATATGGCTTGCGCTATTCTCATAAGGCAGCTTCAGACAGGAGGCATCATTGACACTGTCACAGGGGATAGGATGACTATTGATGAGGCAGTGACCAATAATCTAGTAGCTGCTAAGATTGCTCTTGTAATTCTGGAATCCCTCTGGTCATTTATGGGCTTACTGTTGCCTGAATCTGGAGAGATCCTCCCAATTACAGATGCTCTAGAGCAAGGTATTGTATCTACTGAATTAGCACATAAAATTCTCCATAACCGACAGCAAATTGAGGCTCTTTTTCTACCAACACTCACAGAGATTTGGTCCTGGGAAAAAGCAACAGAGAGTGGTATCCTAGATAAAGATCTTGTCAATAACTTAAGGTCAGTTTGTATCCCTGACATGATGCCCCACATACAACTAGCAGACTCTGCAGAACAAAGCAAGGTAGGCTTTGCTGCTGGTAAACCACCTGTGTCAGGCCCAAGAGAGGAAGGATCCAGCCATGGAGAGAAGCTGCTGTTTCAGCTGATGACTCACAGCTACATTCATGCACACACTGGACAGAGGCTGCTTTTGCTGGATCAAGAGCTGGTGGAGATGCTAACATCCAGAGATGACTGTCAAGTAATTCTTCCAGAAGTATTTGAAATTCAGCATCAAAGGCTCAACACTTCTGAGGCATTACAAGAACTGTATACTGGGACCATCTCCCAAATTTCCAGTGCTAAGCATCCTAGGAAGCCCTGTGAATCCCAATTTCTTTCACAGAACAAAGACTATCCCAGTCAGGAAAACTGTACTGAAGCAAAAGGTGAAAGGTCTGTTGTAGGTATAGAGTGCTCTCCTGCAGAGAGCCCTGAAAGGGAGCTTTTCCTCAAAGAACAAGAAGCCATTATTGAAAATGTTGGCAGTTTGAAGGTCATCAATAAAGTCAAGTTAAAGCTACAAAGACCATTGTTAGGAAGTAGAAAGGAAGAGCAAGCAGAAACATTGAGGGAGGAAAATATCAGCGGAGACCCCCTCCTTGTAGAATGTCCTGAGGAATCTGAAGGTAAAGACCTTTCTACTGAGAAGTCAAAATGCCAAACTCCCACAAAGTGTTCGTTTACATGTCACAAAGAACAAGTGAAGACCATCAAAGACATTCCAAGTGAAACTGGAACATCACTCATAAAGTCTCAAAATCAAATGTCACAATTTCAGGTAGATACTTCTGTAGGTTTGAGATCAGAGTTTAAGTCAGAACATGATATGAATGTTAATTCTTTGGAGAAAGAGTTAAAGGAAGAACTTTTGGTTAAGGATGGCCACAAACAAAGCCAAGAAGGACAGAGTGTTGCAGATGGTCAGACGGTGGCGTTAGAAAAGACTGATACTGAAGATAATGCTGATGAGCCTGCCTTGTTGCATAGTTCTCCTTTTGAAGATGCTACCTTAAGCACACTATCTGCACAGCTACAGGATGGTGGTATCTTTAATGAAGAAACAGGCCAGAAACTCTTACTAAATGAAGCAATAGCCCAAGGCCTTGTGTCTAGCCACACTGCTGTCAAACTTATGGGGAAATTGAACATGTTTCGGGGCTTCTTTGACTCCCAGACCTGTGAGTCTTTGACGACTGAAGAAGTCATTGATGAAGGTCTAATGGATGAGAAATTATTATATAATGTTCTTATGTCAGACAAAGCTATAAGTGGTATCTTAGACCCCAGGACCCACTCTCTGTGCTCTGTAAAGGAGGCTGTGGCAGCTGGGCTGCTTGACAAGGAGACAGCCACAAGGATTTTAGAGGGACAGGTGATAACTGGTGGAATTGTTGACCTGAAACGAGGCAAAAAGCTTTCGGTAACTTTGGCCTCAAATCTTGGTTTGGTAGACACTGCTGACCAGACAGAACTTATCAATCTGGAAAAGGCTACTAAAGGCAGAGGTGCTGAAAAGGCAGTTAAAGAGAGATTAATCGAACTACAAATGGAAACAGCAGGACTTATGGACCCTGAAAGTAAAGCACCGTTAACAGTTTTGCAGTCCATTGATAGAGGTATTCTAGAGAGAGAAGCAGCTGTTTATCTGTTGACTAAGCAAGTACTTGATGGTGGTATCATTCATCATATATCTGGCTTGAGACTTTCTGTTGATAATGCCTTTAAACATGGCTTGATTGGTGAAGATATGGCCAGGCAGCTCAGAAAAGTTGAGAACTTTATCCACTATCAATTTTTCCATCCTCAAACAAAGGAAGCCCTTTCCTTCTCAGAAGCCATAAAATTAGATCTTGTTTCTCCAGACTTGAAAAGAGAAATCCAAGAGATCCAGGACTTTAGTGGGAACTTGGGGGATTTCATTTATGGCCAGAAATTGACCTTGGCAAAAACTAACAAAGAAGAATCCTTAGCTAATAAAACAGAATTGCCTTCAGGGGTGATGCATGGGGTTATAGATCCTGAGAACTGCACGATTATTCCTTACTCAGAATTGGTAAAGAAATGTAGGATTGACACTGAATCTGGATGGCGATATCTTGAAGTAATTCCGTTCTCTGATATTAAAGACGAGGCGGGCAACAACGTGCTCACGCCGCCGGAAGCAATTCAGCTCGGGAAAGTAGACTTTGCTTCTGCATTGAAGGTTTTGGAAGCTCAGGCGAACACTGGTGGAATCATAGACATGGCCACAGGAAAAAGAGTGACATTGGCATCAGCTTTGGAAAAGAAACTGTTAGATGAAAACATGGCCAGGATTATTGCATCTCACCAGATGTTAAGTGGAGGAATTATTGATATATACAGTGATCAAAGAGTGACTTTAAACGATGCTGTCGAGAAAAGACTGATTAGCCCTGAACTGGCAGCTATGATCCAAGTAGATCCCTTAGCAGAGCAAGGTGGGACTGGAGTATGTGAATTGAAGGGTGACTTTCTAAGAAAGGAGTTACTATCTGAGTCTAGTAAGACCCCCAGAGAGAGTTATAGTAAGGAGAAACACGAGGCAGTGTTACAAGCTGGGAGTCTGTGTGCTCCAGAAAAGGCTGGCATAAGGGGTTCTAATGGGGAAAAGGCAGAAAAGGGCAGGAAGATTTCAGTAGAGATGGAAGGCCAGCGTCAAGATGAGAAAGCTTCTTCAGATAATAAAGTATCTGCCAGCATCTTAAGTCCTTTTGGTTTTGAGGGTGAATCTTCATACCAAGTTTCGGTGACGCATCCCTGCTCAGAATCTTGTGATTTGAAACCCAGAGAGGAAACTAGAAGTTGTATGAAAAAATGTGCAGTTGTAGAGCGGGACAAAGTAGTAACACAAATAAAAATGGTTTCTCACGTAAAGCAATCTACCTCAGGTCTGGATGCTGAGGAAGCTAGAGAACGTCAAGGGAGAATGGTTTCAAAAGAGCAAGGATCTCATTATGAAACAGCAGGCAACCTGTTGAGTGAGCGGTCAGTAAGGGTTGATAGAAGAGTGAGGAGAGAGATGGGTGGAGAGCAAAGTGTCCAAATGAGCAGGGAAGCAGCAGTTCTTTCTGAGGAAGAGTTGGACCAGGAAGTTACAATTGGTGATGAACCCGACTCCTTTGTAAAGAGCCAGTCTATGAAAATGATTGGAAATGACAAGGGAAAAGAAGCTGGCATAGAAAAGGACATTTCTGTTGTTTGTAAAATCGAAGGCTTCCCTTCCCAGATGACATCCAAAGATGCTTCCCTCACAAATCAAGATGCTTTACCGTTCTATACTGAAGGGGAAACAAAAACTGTAAACCTCTGCTCGATTTTAAAACCGGGAGAAAAATTATCTCAAGAAACTGCTAGTACTGTCCAGAAAGAACCATTATCTTCTGAGATCCCAAGACCAGAAAGACTGAACTCCCAGGAATCAGATGAAGAACCCCAAATTTCAGATGTACCCCACATTTCCAAAGGAGACATGGCTGCCCAAATTACCACAAGACAGGAGACTACTGATGTCCAAGACCTATATATTACATCTAAGAGTTCAGAGACCAAAGATAAGATTTTCCCATCCAAAAATTATATAGAAAAGTTACATCAAGAAATACCTATGGACCCAACAAGGTCCCATAAATTGAAAGAGGCCACAATTTCTACTCTAGAGACAGAAGGTATCAGTTATCTAGATTCTTCAGATATAAAGAGTCTTTGTGAGGATAGCAAAGCAGACCACAAGTCTTGTGGACATCAGAAATCTAAAGTAACAACAACCCAGGCAAAAAAATCTCTAGAAGTAGTAGATCTCCTAGTTAGAGACACAGAAGAAGGGTCCTCTGAAGATAGAGTGGGCCAAAGAGGACCCAGGGTCTTGGCATCTCTTCTTCCGGAGAAACTGCCCACAAGGACAGTGCAGAGTGAGAATATACGGCAGCATGATGCTGTCATTCCGGCCATTTCTGAAATCAGGGAGGAAATGGCACTGTCGCTGCCATGTTCTGTAGTGAAGGTGGATGGGAAGATACCTAAGGAAAAGCACAAAGAGATCCTTGGAGATGAACAAGGCCCCTTCATGGCCATACCCAGTGGGAAGGGGATTGAAGGTGTAAACCCAGAGCCTTGCAGAGCAACTCAG
Seq C2 exon
ACTCTGCAGAAACAACAAGACACCTGCCATAAGAAGCTGGAGGATCTTTGCAACTGGGTGGGACAGGCAGAAAGAGCACTGGAGAGGCACCAGGGCGGAGCCTCTCGGCAAGAGCTCCCTGCTCTGCAGCAGAACCAAAGTGACTTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'72-70,'72-68,74-70=AN
Average complexity
A_C2
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.222 A=0.300 C2=0.484
Domain overlap (PFAM):
C1:
NO
A:
PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.4),PF0068115=Plectin=WD(100=1.9),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.4),PF0068115=Plectin=WD(100=2.3),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.1)
C2:
NO
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types