Special

MmuEX0027474 @ mm9

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123138373-123157565:-
Coord C1 exon
chr4:123157430-123157565
Coord A exon
chr4:123148250-123153580
Coord C2 exon
chr4:123138373-123138477
Length
5331 bp
Sequences
Splice sites
3' ss Seq
GTTTTAATTCTTTCTTTTAGGGT
3' ss Score
11.63
5' ss Seq
CAGGCAAGT
5' ss Score
3.1
Exon sequences
Seq C1 exon
GCTTTCAGAAGGAGAGAAGGAACAGATCTCTGAGCAGTTGCGTGTGCTGAACAAGACTTACCATGACCTTTGTGATGGTTCTGCAAACCAACTCCAGCAGCTCCAAAGCGAGTTGGCTCAGCAGACGGAACAAAAG
Seq A exon
GGTTGCAGAGCAGTTGCTGGGGTGATTGACCTAGGCACAGTGGAGATATTCCCCATCTTCAGAGCCATGCAAAAGGGCCTCATTGACCAAGACACAGGCCTTGTGCTCCTGGAATCCCAGATTATCATGTCTGGCCTCATTGATCCCGAGAACAGTGAAAAGCTCTCCTTGGAAGAGGGTCTCACCAGAAATTTCATTAACCTTCCAATATATCAACAGCTCTTGGGACTAAGGGATTCTCTGTCTTTAGTAAGCAGGCTTACAGGGACCCTAGGCTCCCTTTCTGTGGTGGAAGCAATTGAAAAGAAAATAATCAGTGAGAGACTTGGACTGAAAGTCTTAGAAGTTCACCTGGCAACTGGAGGTTTCAGTCTCCCCCCTAGTGAGAACTGTATTAATCTGGAAGAAGCTTTTCATCAAGGCTTCATTGCATCAAGTCTTCACTCAGAATTACAGTCTCACCTGAGATCATCCAAGAATTTAATAGACCCCAATACAGCTGAAAAAGTTGGCTTGCTGGACCTGATGCAGAGATGTATCATCCATCAAGAGTCAGGGTTGAAATTGTTGCCTGTCAAGCAGTTGGCAGGGGGAATGGTGAGCTTGAAGTCAGGTCGGAAAGTTAGCATTTTCCGTGCTGTTCAGGAAGGGCTGATAGACAGGCAAGTCACTGTCCGGCTGCTGGAAGCCCAGCTATTTGCTGGTGGCATAGTAGATCCAAGAACAGGACACAGACTTACAGTGGAAGAGGCTGTGAGACATAATTTGATTGACCAAGATATGGCTTGCGCTATTCTCATAAGGCAGCTTCAGACAGGAGGCATCATTGACACTGTCACAGGGGATAGGATGACTATTGATGAGGCAGTGACCAATAATCTAGTAGCTGCTAAGATTGCTCTTGTAATTCTGGAATCCCTCTGGTCATTTATGGGCTTACTGTTGCCTGAATCTGGAGAGATCCTCCCAATTACAGATGCTCTAGAGCAAGGTATTGTATCTACTGAATTAGCACATAAAATTCTCCATAACCGACAGCAAATTGAGGCTCTTTTTCTACCAACACTCACAGAGATTTGGTCCTGGGAAAAAGCAACAGAGAGTGGTATCCTAGATAAAGATCTTGTCAATAACTTAAGGTCAGTTTGTATCCCTGACATGATGCCCCACATACAACTAGCAGACTCTGCAGAACAAAGCAAGGTAGGCTTTGCTGCTGGTAAACCACCTGTGTCAGGCCCAAGAGAGGAAGGATCCAGCCATGGAGAGAAGCTGCTGTTTCAGCTGATGACTCACAGCTACATTCATGCACACACTGGACAGAGGCTGCTTTTGCTGGATCAAGAGCTGGTGGAGATGCTAACATCCAGAGATGACTGTCAAGTAATTCTTCCAGAAGTATTTGAAATTCAGCATCAAAGGCTCAACACTTCTGAGGCATTACAAGAACTGTATACTGGGACCATCTCCCAAATTTCCAGTGCTAAGCATCCTAGGAAGCCCTGTGAATCCCAATTTCTTTCACAGAACAAAGACTATCCCAGTCAGGAAAACTGTACTGAAGCAAAAGGTGAAAGGTCTGTTGTAGGTATAGAGTGCTCTCCTGCAGAGAGCCCTGAAAGGGAGCTTTTCCTCAAAGAACAAGAAGCCATTATTGAAAATGTTGGCAGTTTGAAGGTCATCAATAAAGTCAAGTTAAAGCTACAAAGACCATTGTTAGGAAGTAGAAAGGAAGAGCAAGCAGAAACATTGAGGGAGGAAAATATCAGCGGAGACCCCCTCCTTGTAGAATGTCCTGAGGAATCTGAAGGTAAAGACCTTTCTACTGAGAAGTCAAAATGCCAAACTCCCACAAAGTGTTCGTTTACATGTCACAAAGAACAAGTGAAGACCATCAAAGACATTCCAAGTGAAACTGGAACATCACTCATAAAGTCTCAAAATCAAATGTCACAATTTCAGGTAGATACTTCTGTAGGTTTGAGATCAGAGTTTAAGTCAGAACATGATATGAATGTTAATTCTTTGGAGAAAGAGTTAAAGGAAGAACTTTTGGTTAAGGATGGCCACAAACAAAGCCAAGAAGGACAGAGTGTTGCAGATGGTCAGACGGTGGCGTTAGAAAAGACTGATACTGAAGATAATGCTGATGAGCCTGCCTTGTTGCATAGTTCTCCTTTTGAAGATGCTACCTTAAGCACACTATCTGCACAGCTACAGGATGGTGGTATCTTTAATGAAGAAACAGGCCAGAAACTCTTACTAAATGAAGCAATAGCCCAAGGCCTTGTGTCTAGCCACACTGCTGTCAAACTTATGGGGAAATTGAACATGTTTCGGGGCTTCTTTGACTCCCAGACCTGTGAGTCTTTGACGACTGAAGAAGTCATTGATGAAGGTCTAATGGATGAGAAATTATTATATAATGTTCTTATGTCAGACAAAGCTATAAGTGGTATCTTAGACCCCAGGACCCACTCTCTGTGCTCTGTAAAGGAGGCTGTGGCAGCTGGGCTGCTTGACAAGGAGACAGCCACAAGGATTTTAGAGGGACAGGTGATAACTGGTGGAATTGTTGACCTGAAACGAGGCAAAAAGCTTTCGGTAACTTTGGCCTCAAATCTTGGTTTGGTAGACACTGCTGACCAGACAGAACTTATCAATCTGGAAAAGGCTACTAAAGGCAGAGGTGCTGAAAAGGCAGTTAAAGAGAGATTAATCGAACTACAAATGGAAACAGCAGGACTTATGGACCCTGAAAGTAAAGCACCGTTAACAGTTTTGCAGTCCATTGATAGAGGTATTCTAGAGAGAGAAGCAGCTGTTTATCTGTTGACTAAGCAAGTACTTGATGGTGGTATCATTCATCATATATCTGGCTTGAGACTTTCTGTTGATAATGCCTTTAAACATGGCTTGATTGGTGAAGATATGGCCAGGCAGCTCAGAAAAGTTGAGAACTTTATCCACTATCAATTTTTCCATCCTCAAACAAAGGAAGCCCTTTCCTTCTCAGAAGCCATAAAATTAGATCTTGTTTCTCCAGACTTGAAAAGAGAAATCCAAGAGATCCAGGACTTTAGTGGGAACTTGGGGGATTTCATTTATGGCCAGAAATTGACCTTGGCAAAAACTAACAAAGAAGAATCCTTAGCTAATAAAACAGAATTGCCTTCAGGGGTGATGCATGGGGTTATAGATCCTGAGAACTGCACGATTATTCCTTACTCAGAATTGGTAAAGAAATGTAGGATTGACACTGAATCTGGATGGCGATATCTTGAAGTAATTCCGTTCTCTGATATTAAAGACGAGGCGGGCAACAACGTGCTCACGCCGCCGGAAGCAATTCAGCTCGGGAAAGTAGACTTTGCTTCTGCATTGAAGGTTTTGGAAGCTCAGGCGAACACTGGTGGAATCATAGACATGGCCACAGGAAAAAGAGTGACATTGGCATCAGCTTTGGAAAAGAAACTGTTAGATGAAAACATGGCCAGGATTATTGCATCTCACCAGATGTTAAGTGGAGGAATTATTGATATATACAGTGATCAAAGAGTGACTTTAAACGATGCTGTCGAGAAAAGACTGATTAGCCCTGAACTGGCAGCTATGATCCAAGTAGATCCCTTAGCAGAGCAAGGTGGGACTGGAGTATGTGAATTGAAGGGTGACTTTCTAAGAAAGGAGTTACTATCTGAGTCTAGTAAGACCCCCAGAGAGAGTTATAGTAAGGAGAAACACGAGGCAGTGTTACAAGCTGGGAGTCTGTGTGCTCCAGAAAAGGCTGGCATAAGGGGTTCTAATGGGGAAAAGGCAGAAAAGGGCAGGAAGATTTCAGTAGAGATGGAAGGCCAGCGTCAAGATGAGAAAGCTTCTTCAGATAATAAAGTATCTGCCAGCATCTTAAGTCCTTTTGGTTTTGAGGGTGAATCTTCATACCAAGTTTCGGTGACGCATCCCTGCTCAGAATCTTGTGATTTGAAACCCAGAGAGGAAACTAGAAGTTGTATGAAAAAATGTGCAGTTGTAGAGCGGGACAAAGTAGTAACACAAATAAAAATGGTTTCTCACGTAAAGCAATCTACCTCAGGTCTGGATGCTGAGGAAGCTAGAGAACGTCAAGGGAGAATGGTTTCAAAAGAGCAAGGATCTCATTATGAAACAGCAGGCAACCTGTTGAGTGAGCGGTCAGTAAGGGTTGATAGAAGAGTGAGGAGAGAGATGGGTGGAGAGCAAAGTGTCCAAATGAGCAGGGAAGCAGCAGTTCTTTCTGAGGAAGAGTTGGACCAGGAAGTTACAATTGGTGATGAACCCGACTCCTTTGTAAAGAGCCAGTCTATGAAAATGATTGGAAATGACAAGGGAAAAGAAGCTGGCATAGAAAAGGACATTTCTGTTGTTTGTAAAATCGAAGGCTTCCCTTCCCAGATGACATCCAAAGATGCTTCCCTCACAAATCAAGATGCTTTACCGTTCTATACTGAAGGGGAAACAAAAACTGTAAACCTCTGCTCGATTTTAAAACCGGGAGAAAAATTATCTCAAGAAACTGCTAGTACTGTCCAGAAAGAACCATTATCTTCTGAGATCCCAAGACCAGAAAGACTGAACTCCCAGGAATCAGATGAAGAACCCCAAATTTCAGATGTACCCCACATTTCCAAAGGAGACATGGCTGCCCAAATTACCACAAGACAGGAGACTACTGATGTCCAAGACCTATATATTACATCTAAGAGTTCAGAGACCAAAGATAAGATTTTCCCATCCAAAAATTATATAGAAAAGTTACATCAAGAAATACCTATGGACCCAACAAGGTCCCATAAATTGAAAGAGGCCACAATTTCTACTCTAGAGACAGAAGGTATCAGTTATCTAGATTCTTCAGATATAAAGAGTCTTTGTGAGGATAGCAAAGCAGACCACAAGTCTTGTGGACATCAGAAATCTAAAGTAACAACAACCCAGGCAAAAAAATCTCTAGAAGTAGTAGATCTCCTAGTTAGAGACACAGAAGAAGGGTCCTCTGAAGATAGAGTGGGCCAAAGAGGACCCAGGGTCTTGGCATCTCTTCTTCCGGAGAAACTGCCCACAAGGACAGTGCAGAGTGAGAATATACGGCAGCATGATGCTGTCATTCCGGCCATTTCTGAAATCAGGGAGGAAATGGCACTGTCGCTGCCATGTTCTGTAGTGAAGGTGGATGGGAAGATACCTAAGGAAAAGCACAAAGAGATCCTTGGAGATGAACAAGGCCCCTTCATGGCCATACCCAGTGGGAAGGGGATTGAAGGTGTAAACCCAGAGCCTTGCAGAGCAACTCAG
Seq C2 exon
ACTCTGCAGAAACAACAAGACACCTGCCATAAGAAGCTGGAGGATCTTTGCAACTGGGTGGGACAGGCAGAAAGAGCACTGGAGAGGCACCAGGGCGGAGCCTCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649_MULTIEX1-1/5=C1-C2
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.294 A=0.300 C2=0.357
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.4),PF0068115=Plectin=WD(100=1.9),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.4),PF0068115=Plectin=WD(100=2.3),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.1)

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000081324f2416A





     
                   









    
Main Skipping Isoform:
ENSMUSP00000101827





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000081324, ENSMUSP00000081324f6186A, ENSMUSP00000101819, ENSMUSP00000101831, ENSMUSP00000101831f2417A
          









    
Other Skipping Isoforms:
ENSMUSP00000080755, ENSMUSP00000095508, ENSMUSP00000095509, ENSMUSP00000114568, ENSMUSP00000115847, ENSMUSP00000119885, ENSMUSP00000123246
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
chr1:39331203-39336653 
ALTERNATIVE
HsaEX0037170
(MACF1)
Human
(hg19)
chr1:39796875-39802325 
ALTERNATIVE
HsaEX0037170
(MACF1)
Rat
(rn6)
chr5:141157139-141162457 
ALTERNATIVE
RnoEX6023208
(Macf1)
Cow
(bosTau6)
chr3:107298483-107303852 
ALTERNATIVE
BtaEX0021021
(MACF1)
Chicken
(galGal4)
chr23:5341431-5345067 
Chicken
(galGal3)
chr23:5656607-5660533 
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
No suggested primer sequences

				
R: 
No suggested primer sequences

				
Band lengths: 

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






Retrieved from "https://vastdb.crg.eu/wiki/Special:VastDB"