Special

MmuEX0027475 @ mm9

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123138373-123157565:-
Coord C1 exon
chr4:123157430-123157565
Coord A exon
chr4:123146966-123147115
Coord C2 exon
chr4:123138373-123138477
Length
150 bp
Sequences
Splice sites
3' ss Seq
GTTTTACTTTGGCTCAACAGAAT
3' ss Score
7.21
5' ss Seq
AAGGTAAGC
5' ss Score
10.22
Exon sequences
Seq C1 exon
GCTTTCAGAAGGAGAGAAGGAACAGATCTCTGAGCAGTTGCGTGTGCTGAACAAGACTTACCATGACCTTTGTGATGGTTCTGCAAACCAACTCCAGCAGCTCCAAAGCGAGTTGGCTCAGCAGACGGAACAAAAG
Seq A exon
AATGTGTTTACTCGGAGACTTTGTTTAGAACATGATGAGAAATTGGTCTCCTATCTGTCTCTGTTACGGGACATTGAAATGAGAACCAAACAGATTCAACCTTTGGAGCTTAATGTGGCAGAGCTGCAAGATCTACTGGGTCAGGCAAAG
Seq C2 exon
ACTCTGCAGAAACAACAAGACACCTGCCATAAGAAGCTGGAGGATCTTTGCAACTGGGTGGGACAGGCAGAAAGAGCACTGGAGAGGCACCAGGGCGGAGCCTCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649_MULTIEX1-2/5=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.294 A=0.000 C2=0.357
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000081324f2416A





     
                   









    
Main Skipping Isoform:
ENSMUSP00000101827





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000081324, ENSMUSP00000081324f6186A, ENSMUSP00000101819, ENSMUSP00000101831, ENSMUSP00000101831f2417A
          









    
Other Skipping Isoforms:
ENSMUSP00000080755, ENSMUSP00000095508, ENSMUSP00000095509, ENSMUSP00000114568, ENSMUSP00000115847, ENSMUSP00000119885, ENSMUSP00000123246
  



Associated events







Other assemblies


mm10




Conservation


Human
(hg38)
chr1:39337182-39337331 
HIGH PSI
HsaEX0037171
(MACF1)
Human
(hg19)
chr1:39802854-39803003 
ALTERNATIVE
HsaEX0037171
(MACF1)
Rat
(rn6)
chr5:141155828-141155977 
ALTERNATIVE
RnoEX6023209
(Macf1)
Cow
(bosTau6)
chr3:107297616-107297765 
ALTERNATIVE
BtaEX0021022
(MACF1)
Chicken
(galGal4)
chr23:5347043-5347192 
Chicken
(galGal3)
chr23:5660996-5661145 
Zebrafish
(danRer10)
chr19:35555540-35555695 
ALTERNATIVE
DreEX6073703
(macf1a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GCTTTCAGAAGGAGAGAAGGAACA

				
R: 
GTGCCTCTCCAGTGCTCTTTC

				
Band lengths: 
226-376

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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