BtaEX0021022 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021024 | MACF1
Description
microtubule-actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]
Coordinates
chr3:107291970-107307769:-
Coord C1 exon
chr3:107307634-107307769
Coord A exon
chr3:107297616-107297765
Coord C2 exon
chr3:107291970-107292119
Length
150 bp
Sequences
Splice sites
3' ss Seq
TTTTCTTTTTGGCTCCACAGAAT
3' ss Score
10.37
5' ss Seq
AAGGTAGGT
5' ss Score
10.29
Exon sequences
Seq C1 exon
GCTGTCAGAAAAAGAGAAAGAACAGATATCTGAGCAATTGAATGCCCTCAGTAAGACTTACCATGATCTTTGTGAAGGTTCAGCAAACCAGCTTCAGCAACTCCAGAGCCAGCTGGCCCAACAGACAGAACAAAAG
Seq A exon
AATGTATTTACCCGGCAGCTCTGCCTAGAACATGATGAGAAGTTGGTATCCTATCTGTCTCTTTTACGGGACATTGAAATGAGGACCAAGCAGATTCAACCTCTAGAGCTCAACCTGGCAGAGCTACAGGATCTGCTGTGTCAGGCCAAG
Seq C2 exon
ACCCTGCAGAAACAACAAAATACCTGTCACCAGAAACTGGAGGATCTTTGCAGCTGGGTGGGACAGGCAGAAAGAGCATTGGCTGGCCATCAGGGTAGAGCCACCCAGCAGGATCTCTCTGCTTTGCAGAAGAACCAAAGTGACCTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021024_MULTIEX1-2/5=C1-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.196 A=0.000 C2=0.520
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0043516=Spectrin=PU(40.7=92.0)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCCCTCAGTAAGACTTACCA
R:
TCAGGTCACTTTGGTTCTTCTGC
Band lengths:
242-392
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development