MmuEX0027475 @ mm10
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123465859-123476337:-
Coord C1 exon
chr4:123471007-123476337
Coord A exon
chr4:123469723-123469872
Coord C2 exon
chr4:123465859-123466074
Length
150 bp
Sequences
Splice sites
3' ss Seq
GTTTTACTTTGGCTCAACAGAAT
3' ss Score
7.21
5' ss Seq
AAGGTAAGC
5' ss Score
10.22
Exon sequences
Seq C1 exon
AATTGAAAGAGGCCACAATTTCTACTCTAGAGACAGAAGGTATCAGTTATCTAGATTCTTCAGATATAAAGAGTCTTTGTGAGGATAGCAAAGCAGACCACAAGTCTTGTGGACATCAGAAATCTAAAGTAACAACAACCCAGGCAAAAAAATCTCTAGAAGTAGTAGATCTCCTAGTTAGAGACACAGAAGAAGGGTCCTCTGAAGATAGAGTGGGCCAAAGAGGACCCAGGGTCTTGGCATCTCTTCTTCCGGAGAAACTGCCCACAAGGACAGTGCAGAGTGAGAATATACGGCAGCATGATGCTGTCATTCCGGCCATTTCTGAAATCAGGGAGGAAATGGCACTGTCGCTGCCATGTTCTGTAGTGAAGGTGGATGGGAAGATACCTAAGGAAAAGCACAAAGAGATCCTTGGAGATGAACAAGGCCCCTTCATGGCCATACCCAGTGGGAAGGGGATTGAAGGTGTAAACCCAGAGCCTTGCAGAGCAACTCAG
Seq A exon
AATGTGTTTACTCGGAGACTTTGTTTAGAACATGATGAGAAATTGGTCTCCTATCTGTCTCTGTTACGGGACATTGAAATGAGAACCAAACAGATTCAACCTTTGGAGCTTAATGTGGCAGAGCTGCAAGATCTACTGGGTCAGGCAAAG
Seq C2 exon
GAACTTGACAGGGAATTAAAAGATCTGTCCACTGTGGTGAGTCAGGAATTGGAGTGTGTGGATCGGATTGTTATCAGTCAGCCACAAGAAGTTCCTGCTCAGCTGTTGAAGGCATTAGAGAAAGATGCCAAAAATCTTCAGAAGTCTCTAGACTCTGTTAGTGACTCTTGGAGTTCCAGATTCCTCCACCTCCAGAGTGCTGTGGAAGTAAAAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'74-71,'74-70,75-71
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.300 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.4),PF0068115=Plectin=WD(100=1.9),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.4),PF0068115=Plectin=WD(100=2.3),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.1)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGCTGTCATTCCGGCCATTT
R:
TTCCTGACTCACCACAGTGGA
Band lengths:
245-395
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types