MmuEX0027478 @ mm9
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123138373-123157565:-
Coord C1 exon
chr4:123157430-123157565
Coord A exon
chr4:123140781-123141014
Coord C2 exon
chr4:123138373-123138477
Length
234 bp
Sequences
Splice sites
3' ss Seq
TCTCTCCCCATTTGCCTTAGGAT
3' ss Score
9.84
5' ss Seq
AAGGTCAGA
5' ss Score
6.8
Exon sequences
Seq C1 exon
GCTTTCAGAAGGAGAGAAGGAACAGATCTCTGAGCAGTTGCGTGTGCTGAACAAGACTTACCATGACCTTTGTGATGGTTCTGCAAACCAACTCCAGCAGCTCCAAAGCGAGTTGGCTCAGCAGACGGAACAAAAG
Seq A exon
GATATGAAACAATCTATGGCTGAAAGGAAATCTCAGCTGGATGCCCTTGCTTTGGATATTCAGCTCTTTATATCTGAGCACCCACAGGATCTGTCTCTTCAGCAGAATCAGGAGATGCTGCAGTTTCTGAGTGAATTACAGAGGTCCTTCCAGGGCCTTGTGGAACACACTGCAGCTCAGAAAGATGTGGTGCAGGGCCATCTTCAGCAAGTCCAGCAAGAAGTTCAGGTCAAG
Seq C2 exon
ACTCTGCAGAAACAACAAGACACCTGCCATAAGAAGCTGGAGGATCTTTGCAACTGGGTGGGACAGGCAGAAAGAGCACTGGAGAGGCACCAGGGCGGAGCCTCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649_MULTIEX1-5/5=C1-C2
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.294 A=0.128 C2=0.357
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Other Inclusion Isoforms:
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTTTCAGAAGGAGAGAAGGAACA
R:
CTGGTGCCTCTCCAGTGCTC
Band lengths:
229-463
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: