Special

MmuEX0033217 @ mm9

Exon Skipping

Gene
ENSMUSG00000029822 | Osbpl3
Description
oxysterol binding protein-like 3 [Source:MGI Symbol;Acc:MGI:1918970]
Coordinates
chr6:50270036-50277467:-
Coord C1 exon
chr6:50277374-50277467
Coord A exon
chr6:50272972-50273222
Coord C2 exon
chr6:50270036-50270173
Length
251 bp
Sequences
Splice sites
3' ss Seq
TCAGGCTTCCTTCCTTTCAGGGC
3' ss Score
9.92
5' ss Seq
ATGGTGAGG
5' ss Score
7.61
Exon sequences
Seq C1 exon
ATTTCTGATGATGATTCATACGTCAGTGACATAAGTGATAATCTGTCCTTAGACAACCTCAGTAATGATTTAGACAATGAAAGACAGACCTTGG
Seq A exon
GGCCTGTTCTCGAGAGTAGTGGGGAAGCCAGATCCAAAAGGAGGACCAGCTTGCCGGCACCGGGTCCCAACACCAGTAGCGTTAGCTTGTGGAGCATCCTGCGCAACAACATTGGGAAGGACCTGTCCAAGGTGGCCATGCCGGTGGAGCTGAACGAGCCCCTGAACACGCTGCAGCGGCTCTGCGAGGAGCTGGAATACAGCGAGCTTCTGGACAAGGCCTCGAGAATCCCCAGTCCCCTGGAGAGGATG
Seq C2 exon
GTGTACGTAGCAGCCTTCGCCATCTCCGCATATGCATCCAGCTACTTCCGAGCAGGCAGCAAGCCGTTTAACCCGGTCCTTGGAGAAACGTACGAATGCATCCGACAGGACAAGGGTTTCCAGTTTTTTGCCGAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000029822-'23-24,'23-23,24-24
Average complexity
S
Mappability confidence:
88%=100=75%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.719 A=0.262 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
PF0123713=Oxysterol_BP=PU(15.9=65.5)

							
C2:
PF0123713=Oxysterol_BP=FE(43.3=100),PF0123713=Oxysterol_BP=PU(0.1=0.0)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000087473





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000071643, ENSMUSP00000110110, ENSMUSP00000110112, ENSMUSP00000110114
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr7:24834087-24834212 
ALTERNATIVE
HsaEX6007529
(OSBPL3)
Human
(hg19)
chr7:24874105-24874355 
HIGH PSI
HsaEX6007529
(OSBPL3)
Rat
(rn6)
chr4:80055272-80055386 
Cow
(bosTau6)
chr4:71456341-71456591 
HIGH PSI
BtaEX6027820
(OSBPL3)
Chicken
(galGal4)
chr2:31793536-31793786 
HIGH PSI
GgaEX1041410
(OSBPL3)
Chicken
(galGal3)
chr2:31589728-31589978 
Zebrafish
(danRer10)
chr16:21562199-21562443 
HIGH PSI
DreEX0052763
(osbpl3b)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TTTCTGATGATGATTCATACGTCAGT

				
R: 
AAAACTGGAAACCCTTGTCCTGT

				
Band lengths: 
220-471

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






Retrieved from "https://vastdb.crg.eu/wiki/Special:VastDB"