MmuEX0038804 @ mm9
Exon Skipping
Gene
ENSMUSG00000041992 | Rapgef5
Description
Rap guanine nucleotide exchange factor (GEF) 5 [Source:MGI Symbol;Acc:MGI:2444365]
Coordinates
chr12:118896751-118927709:+
Coord C1 exon
chr12:118896751-118896876
Coord A exon
chr12:118927037-118927155
Coord C2 exon
chr12:118927621-118927709
Length
119 bp
Sequences
Splice sites
3' ss Seq
ACTCTGATCTACAATTAAAGCAA
3' ss Score
3.26
5' ss Seq
GAGGTGAGA
5' ss Score
7.66
Exon sequences
Seq C1 exon
GCTGGGGTGATGTGCAAGCTTCAAGAGAGAGATGATATCGGACGTATTGAGCTGGTCCACAAGCTGGCAAGAGAAAACTGTCAGTTTTTACAGACAGAAAAAAAAGAATCGGAGAAGTTGGAACAG
Seq A exon
CAAGATGATGAAGTGACGATGGTTCAGGTTAAAGAGCAAGGCCAGAGTGTGCTGGTGCTGAAGAAAGTAGCAAGCTGTGGCCCAGCCCCCACCTCAGGGAGTGCGGAGAACGACGCGAG
Seq C2 exon
ATATGTGGTGGTGTCTGGTACCCCGGAGAAGATCTTGGAGCACCTTTTGAATGACTTGCACCTGGCAGAGGTTCAGCACAAAGAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000041992-'12-14,'12-13,14-14
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.357 A=0.375 C2=0.032
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0061815=RasGEF_N=PU(25.5=90.3)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGGGGTGATGTGCAAGCTTC
R:
TTCTTTGTGCTGAACCTCTGCC
Band lengths:
210-329
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: