MmuEX0038891 @ mm10
Exon Skipping
Gene
ENSMUSG00000004952 | Rasa4
Description
RAS p21 protein activator 4 [Source:MGI Symbol;Acc:MGI:1858600]
Coordinates
chr5:136091748-136096792:+
Coord C1 exon
chr5:136091748-136091809
Coord A exon
chr5:136093138-136093267
Coord C2 exon
chr5:136096661-136096792
Length
130 bp
Sequences
Splice sites
3' ss Seq
ACTGACATAAAGTCCTGCAGGAT
3' ss Score
0.55
5' ss Seq
GAGGTGAGA
5' ss Score
7.66
Exon sequences
Seq C1 exon
CCGGGACGATGTGATCGGGAAGGTCTGCCTCACGAGGGACGCGCTAGCCTCTCACCCAAAGG
Seq A exon
GATTCAGTGGATGGACCCACCTGGTGGAGGTGGACCCGAACGAGGAGGTGCAGGGTGAGATCCACCTGCGCCTGGAGGTGGTGCCAGGGGTCCATGCCAGCCGGCTGCGCTGCGCGGTGCTTGAGGCGAG
Seq C2 exon
GTGGTGAAGAAATCCTGCTACCCACGCTGGAATGAGACATTTGATTTTGAGCTGGAAAAAGGAGCCTCAGAAGCACTGCTGGTGGAGGCCTGGGACTGGGATCTTGTTAGCCGGAATGACTTTCTAGGCAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000004952_MULTIEX3-1/2=C1-C2
Average complexity
C2*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0016825=C2=PD(11.0=40.9)
A:
PF0016825=C2=PU(9.8=18.2)
C2:
PF0016825=C2=FE(52.4=100)
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGTGATCGGGAAGGTCTGC
R:
TTGCCTAGAAAGTCATTCCGG
Band lengths:
186-316
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types