Special

MmuEX0048653 @ mm9

Exon Skipping

Gene
ENSMUSG00000033200 | Tpsg1
Description
tryptase gamma 1 [Source:MGI Symbol;Acc:MGI:1349391]
Coordinates
chr17:25506218-25510815:+
Coord C1 exon
chr17:25506218-25506362
Coord A exon
chr17:25510120-25510385
Coord C2 exon
chr17:25510670-25510815
Length
266 bp
Sequences
Splice sites
3' ss Seq
CATCTCTCCCTGCCTCCCAGGTC
3' ss Score
9.59
5' ss Seq
GAGGTAAGG
5' ss Score
10.28
Exon sequences
Seq C1 exon
AGTTCAGCCCAGCCCAGCCATCAGGTGACAGATAAAAACGGTCATTGGATTCATCTCTGCCATGGGCTACAGGGACCAGGCTTAGCTGAGAGTTTTCAGTGGGCAACATCCAAACCCACTGTCTCAGCCAGAGGCCAGTACCCAG
Seq A exon
GTCTGTGAACTCGTCTGATTATCAGGTGCACTTGGGAGAGCTTACGGTCACACTGTCTCCCCACTTCTCCACTGTAAAACGGATCATCATGTACACTGGCTCTCCAGGACCACCGGGGTCCAGTGGGGACATTGCCCTGGTGCAGCTGTCCTCCCCGGTGGCCCTTTCCAGCCAGGTCCAGCCTGTGTGCCTCCCAGAGGCCTCAGCTGACTTCTACCCTGGGATGCAGTGCTGGGTGACTGGCTGGGGCTATACAGGGGAGGGAG
Seq C2 exon
AGCCTCTGAAGCCCCCATACAACCTTCAGGAGGCCAAAGTCTCTGTGGTGGATGTAAAGACCTGCAGCCAGGCTTACAATAGTCCCAATGGCAGCCTCATCCAGCCAGACATGCTATGCGCCCGGGGCCCTGGGGATGCCTGCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000033200_MULTIEX1-2/2=C1-C2
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=NA A=0.107 C2=0.047
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NA

							
A:
PF0008921=Trypsin=PU(48.1=97.8)

							
C2:
PF0008921=Trypsin=PU(19.0=91.7)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000124008





     
                   









    
Main Skipping Isoform:
ENSMUSP00000125180





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000024999, ENSMUSP00000123828, ENSMUSP00000124721
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr16:1222652-1222917 
HIGH PSI
HsaEX6041529
(TPSG1)
Human
(hg19)
chr16:1272652-1272917 
HIGH PSI
HsaEX6041529
(TPSG1)
Rat
(rn6)
chr10:14729892-14730058 
ALTERNATIVE
RnoEX0093113
(Tpsg1)
Cow
(bosTau6)
chr25:2274320-2274481 
Chicken
(galGal4)
chr1:50271646-50271896 
Chicken
(galGal3)
chr28:610903-611041 
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
AGTTCAGCCCAGCCCAGC

				
R: 
CTGGCAGGCATCCCCAGG

				
Band lengths: 
291-557

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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