MmuEX1029253 @ mm10
Exon Skipping
Gene
ENSMUSG00000057003 | Myh4
Description
myosin, heavy polypeptide 4, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339713]
Coordinates
chr11:67250501-67251948:+
Coord C1 exon
chr11:67250501-67250637
Coord A exon
chr11:67250752-67251007
Coord C2 exon
chr11:67251706-67251948
Length
256 bp
Sequences
Splice sites
3' ss Seq
CTGTTTTTCTCCTTCCACAGAGA
3' ss Score
11.62
5' ss Seq
GCTGTGAGT
5' ss Score
7.39
Exon sequences
Seq C1 exon
GTTTTCTTTAAAGCCGGCCTGTTGGGAACTCTAGAGGAGATGCGAGATGAAAAGCTGGCTCAGCTCATCACCCGCACTCAAGCCGTGTGCAGAGGGTACCTGATGAGGGTGGAGTTCAAAAAGATGATGGAGAGGAG
Seq A exon
AGAGTCCATCTTCTGCATTCAGTACAACGTCCGCGCCTTCATGAATGTGAAACACTGGCCCTGGATGAAGCTGTATTTCAAGATCAAACCTCTGCTGAAGAGCGCGGAGACCGAGAAGGAGATGGCCAACATGAAGGAAGATTTTGAGAAGGCCAAGGAGGATCTGGCTAAGTCAGAGGCAAAAAGGAAGGAACTAGAAGAAAAGATGGTAGCTTTGATGCAGGAGAAAAATGACCTGCAGCTCCAGGTTCAAGCT
Seq C2 exon
GAAGCAGATGGCTTGGCTGATGCTGAGGAAAGGTGCGACCAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGCTGACCGAGAGGGCCGAGGACGAGGAGGAGATCAACGCGGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCTGAAGAAGGACATTGACGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057003-'33-26,'33-23,37-26
Average complexity
S
Mappability confidence:
100%=75=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.140 C2=0.395
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(0.4=6.5)
A:
PF0157614=Myosin_tail_1=PU(28.5=64.0)
C2:
PF0157614=Myosin_tail_1=FE(41.5=100),PF0157614=Myosin_tail_1=PU(29.1=48.1)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGAGATGAAAAGCTGGCTCA
R:
CAGTGTCAGCTCAAGGTCGTC
Band lengths:
300-556
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types