MmuEX6009507 @ mm9
Exon Skipping
Gene
ENSMUSG00000054808 | Actn4
Description
actinin alpha 4 [Source:MGI Symbol;Acc:MGI:1890773]
Coordinates
chr7:29690244-29696594:-
Coord C1 exon
chr7:29696509-29696594
Coord A exon
chr7:29691991-29692083
Coord C2 exon
chr7:29690244-29690474
Length
93 bp
Sequences
Splice sites
3' ss Seq
TGTGTGCTTCTCCACTCTAGGCT
3' ss Score
10.34
5' ss Seq
GATGTGAGT
5' ss Score
7.77
Exon sequences
Seq C1 exon
ACATCGTGAACACAGCCCGGCCCGACGAGAAGGCCATAATGACATATGTGTCCAGCTTCTACCATGCCTTTTCAGGAGCGCAGAAG
Seq A exon
GCTGAGACCGCTGCCAACCGGATCTGCAAGGTGCTGGCTGTGAACCAGGAAAATGAGCACCTGATGGAAGACTATGAACGCCTGGCCAGTGAT
Seq C2 exon
CTTCTAGAGTGGATTCGGCGCACCATCCCCTGGCTGGAGGACCGGGTGCCTCAGAAGACCATCCAGGAAATGCAGCAGAAGCTGGAGGACTTCCGAGACTATAGGCGTGTGCACAAGCCGCCCAAGGTGCAGGAGAAGTGTCAGCTGGAGATCAACTTCAACACACTGCAGACCAAGCTGCGGCTCAGCAACCGGCCTGCCTTCATGCCCTCCGAGGGCAGGATGGTCTCC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000054808-'14-18,'14-17,19-18=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (disopred):
C1=0.138 A=0.296 C2=0.097
Domain overlap (PFAM):
C1:
PF0030726=CH=PD(23.1=82.8)
A:
PF0043516=Spectrin=PU(9.9=35.5)
C2:
PF0043516=Spectrin=FE(68.5=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTCCAGCTTCTACCATGCC
R:
GACACTTCTCCTGCACCTTGG
Band lengths:
181-274
Functional annotations
There are 3 annotated functions for this event
PMID: 16980305
This study isolated a novel splice variant of alpha-actinin 4 (skipping exons 3-12; ENST00000424234)... [Show more]
PMID: 22908231
ACTN4(Iso) overexpression increases transcriptional activity of the VDRE and ERE promoters. Both ACT... [Show more]
PMID: 27998979
Overexpression of either ATN4(WT) or ACTN4(Iso) increases GRE reporter activity. Disruption of the L... [Show more]
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: