MmuEX6015925 @ mm10
Exon Skipping
Gene
ENSMUSG00000063531 | Sema3e
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E [Source:MGI Symbol;Acc:MGI:1340034]
Coordinates
chr5:14225553-14230251:+
Coord C1 exon
chr5:14225553-14226530
Coord A exon
chr5:14226635-14226704
Coord C2 exon
chr5:14230107-14230251
Length
70 bp
Sequences
Splice sites
3' ss Seq
ATGGTTTTTTATTCTTTCAGAGG
3' ss Score
8.26
5' ss Seq
CAGGTGAGA
5' ss Score
9.22
Exon sequences
Seq C1 exon
CACTGCACTGAGGGGTTGAAGACATAGGTTAGCAGTTAAGAGTACTTGCTGCATTTGCGAAGACCTCAATTTACTTCCTAGCACCCACGCCCAACAGTTCGTTACCACCTGTGCGACTCCAAGTCTGTGGCTCCAGTGACCTCTCTTGGCTGGAGCACCGCCTCATATACGGTGAAACTCAACCTAAACAACCTTTGTTTTAAAAAGTACTGCATTGATAAGACAACGCAAGGGAGAAGTGGGAAGGCAGTATTTGCTTTCTGAGAACTTTGCTCAACTTTCCTATGTAAATAAAAAGAGCCACAGCGTCTGCTCGTTTCGCCATATTAAACCTTGGTTGGTAGATGCCCTTGAAGTCCTTGTTTGTCCATTTTGTTTTTCACAGAATGACATGGGAGGACAGAGAATCCTGGTGAACAAGTGGAGCACTTTCCTTAAAGCGCGGCTGGTTTGCTCAGTGCCGGGAATGAATGGAATCGACACATACTTTGACGAACTAG
Seq A exon
AGGATGTGTTTTTACTGCCGACCAGAGATCCTAAGAATCCAGTGATATTTGGACTGTTTAATACTACCAG
Seq C2 exon
CAATATATTTAGAGGCCATGCTGTATGTGTGTATCACATGTCAAGTATCCGGGAAGCCTTTAATGGCCCATATGCTCATAAAGAAGGCCCTGAATACCACTGGTCACTATATGAAGGAAAAGTCCCCTACCCAAGGCCTGGTTCC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000063531-'14-15,'14-14,15-15
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(10.6=100)
A:
PF0140314=Sema=FE(5.2=100)
C2:
PF0140314=Sema=FE(10.8=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGTGCCGGGAATGAATGGAA
R:
GCCTTGGGTAGGGGACTTTTC
Band lengths:
182-252
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types