MmuEX6025757 @ mm9
Exon Skipping
Gene
ENSMUSG00000067158 | Col4a4
Description
collagen, type IV, alpha 4 [Source:MGI Symbol;Acc:MGI:104687]
Coordinates
chr1:82492346-82494509:-
Coord C1 exon
chr1:82494403-82494509
Coord A exon
chr1:82493729-82493912
Coord C2 exon
chr1:82492346-82492414
Length
184 bp
Sequences
Splice sites
3' ss Seq
ATGTAACCTAACATCCCCAGGGG
3' ss Score
4.81
5' ss Seq
AAGGTACTT
5' ss Score
8.4
Exon sequences
Seq C1 exon
GGCAAAAAGGAGAAAGAGGACTGGATGGGCCACCAGGATTTCCGGGGCCACATGGACAAGATGGTGGGGATGGACGTCCTGGAGAAAGAGGGGATCCTGGGCCACGA
Seq A exon
GGGGACCATAAGGATGCAGCCCCGGGTGAGAGAGGGCTTCCCGGACTGCCAGGCCCTCCAGGAAGAACAGGACCTGAGGGGCCTCCAGGCCTGGGATTTCCAGGCCCACCAGGACAGCGAGGCCTACCCGGAGAGCCCGGGCGTCCGGGCACCAGAGGCTTTGATGGCACGAAGGGGCAGAAAG
Seq C2 exon
GTGACTCCATTCTGTGTAATGTAAGCTACCCGGGAAAACCAGGTCTGCCAGGTCTTGATGGACCTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000067158-'26-26,'26-25,27-26=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=1.000
Domain overlap (PFAM):
C1:
PF0139113=Collagen=FE(76.1=100)
A:
PF0139113=Collagen=PD(10.9=8.1),PF0139113=Collagen=PU(93.5=93.5)
C2:
PF0139113=Collagen=PD(4.8=12.5),PF0139113=Collagen=PU(21.7=62.5)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCAAAAAGGAGAAAGAGGACTG
R:
GTCCATCAAGACCTGGCAGAC
Band lengths:
170-354
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: