MmuEX6035197 @ mm10
Exon Skipping
Gene
ENSMUSG00000031491 | Chrna6
Description
cholinergic receptor, nicotinic, alpha polypeptide 6 [Source:MGI Symbol;Acc:MGI:106213]
Coordinates
chr8:27403212-27408424:-
Coord C1 exon
chr8:27408315-27408424
Coord A exon
chr8:27406495-27407473
Coord C2 exon
chr8:27403212-27404522
Length
979 bp
Sequences
Splice sites
3' ss Seq
GTGTGTGGTTTCATTTCCAGTGC
3' ss Score
9.73
5' ss Seq
GAGGTAAAA
5' ss Score
7.3
Exon sequences
Seq C1 exon
ATCTGGAAGGACTACAGATTGCGTTGGGATCCAACGGAGTATGATGGCATCGAGACACTTCGAGTTCCAGCAGACAACATCTGGAAGCCTGACATCGTTCTGTATAACAA
Seq A exon
TGCTGTCGGCGACTTCCAGGTCGAAGGCAAGACCAAAGCTCTTCTCAAGTATGACGGTGTGATAACCTGGACCCCACCAGCCATCTTTAAGAGCTCCTGCCCAATGGACATCACCTTCTTCCCGTTTGACCATCAAAACTGCTCCCTGAAGTTTGGTTCCTGGACTTATGACAAGGCAGAAATCGACCTTCTCATCATTGGCTCTAAAGTAGACATGAACGACTTTTGGGAAAACAGTGAATGGGAAATTGTCGACGCCTCTGGCTATAAGCATGACATCAAGTACAACTGCTGTGAAGAGATTTACACGGACATAACCTACTCCTTCTACATCAGGAGGTTGCCCATGTTTTACACCATCAACCTCATCATCCCCTGCCTCTTCATTTCCTTCCTCACGGTGCTGGTTTTTTACCTTCCCTCCGACTGTGGCGAGAAAGTGACTCTTTGCATCTCCGTTCTGCTTTCTCTCACTGTCTTTTTGCTGGTGATTACAGAGACCATCCCATCCACATCTCTCGTGATCCCACTGGTGGGTGAGTATCTACTGTTCACCATGATCTTTGTCACGCTGTCCATTGTGGTGACCGTGTTCGTGCTGAACATACACTACAGGACCCCAGCAACGCATACCATGCCCAAGTGGGTGAAGACCATCTTCCTTCAGGCCTTCCCCTCGATTCTGATGATGAGGAAACCTCTGGACAAGACAAAGGAGGCAGGAGGTGTTAAGGACCCCAAAAGCCATACCAAGAGGCCTGCCAAGGTCAAATTTACTCATCGAGGAGAATCCAAACTTCTAAAGGAATGCCACCACTGCCAAAAATCAAGTGACATAGCACCTGGAAAGAGAAGATCAAGCCAGCAGCCTGCACGGTGGGTGGCAGAGAATTCAGAGCACTCGTCCGATGTTGAAGATGTCATCGAGAGTGTTCAATTCATAGCAGAAAACATGAAGAGCCACAATGAAACAAACGAG
Seq C2 exon
GTAGAAGACGACTGGAAATACATGGCTATGGTGGTGGACAGAGTCTTCCTTTGGGTATTTATAATTGTCTGTGTGTTTGGAACTGTGGGGCTATTTCTGCAGCCACTGCTTGGGAACACAGGAAAGTCTTAATTGGTATTGTCCCTTTACCTTCTGAATCTTTTAGATGTTGTGTTTTTTCCCGAGCTAGTCCCCACAAGAAAAAGCCAAGTACACAGACTCCTAAACCAAGACAAGAAGGGAGTGGGCATTGCTGCAGCCTTAGGAGCCTGAATACCTGTCTTCCTTACACTGGGTGTTTTATCACCTTGGAGCCCAGAGCTGGCAGTTGTCCTAGGCTAGGAGGACTGAGTCAGCTTCTCTATAATTTGCATTCTTTGTTTCAAGAAACACAAATGGTCCCTCGTAGAGCTAGGCCCCATAGTCAAGAGTCAGTCCACTCTGAAAGGTGATTGGCTTGTATCCGAAGCTCCTGCTGGTTATTGCAAGTGATGTGTATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000031491-'4-5,'4-4,6-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.119 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(17.4=100)
A:
PF0293118=Neur_chan_LBD=PD(55.6=35.2),PF0293211=Neur_chan_memb=PU(86.1=62.4)
C2:
PF0293211=Neur_chan_memb=PD(13.1=70.5)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGATTGCGTTGGGATCCAA
R:
GCCCACTCCCTTCTTGTCTTG
Band lengths:
346-1325
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types