MmuEX6056467 @ mm9
Exon Skipping
Gene
ENSMUSG00000023022 | Lima1
Description
LIM domain and actin binding 1 [Source:MGI Symbol;Acc:MGI:1920992]
Coordinates
chr15:99613951-99627230:-
Coord C1 exon
chr15:99627173-99627230
Coord A exon
chr15:99624840-99624949
Coord C2 exon
chr15:99613951-99614084
Length
110 bp
Sequences
Splice sites
3' ss Seq
GCCTTCTGTGACTTAACCAGGTA
3' ss Score
8.29
5' ss Seq
AAGGTACGG
5' ss Score
10.26
Exon sequences
Seq C1 exon
GTTTCTACAACTGAGAATAGTCTAGTGGCCCTCTCTGTCCCTGCTGAAGATGACACCT
Seq A exon
GTAACTCCCAGGTGAAGAGCGAGGCCCAGCAGCCTATGCACCCTAAGCCGCTGAGTCCTGACGCCAGGACCTCCAGTCTTCCGGAAAGTTCTCCTTCCAAAACAGCGAAG
Seq C2 exon
AAGTTTCAGGCGCCGGCAAAGGAGAGCTGCGTGGAGTGTCAGAAGACGGTGTACCCCATGGAACGGCTCCTGGCCAACCAGCAGGTGTTTCACATCAGCTGTTTCCGATGCTCCTACTGCAACAACAAGCTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000023022-'14-16,'14-13,15-16=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.700 A=1.000 C2=0.044
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0041217=LIM=PU(61.4=77.8)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAACTGAGAATAGTCTAGTGGCC
R:
CTGAGCTTGTTGTTGCAGTAGGA
Band lengths:
186-296
Functional annotations
There are 1 annotated functions for this event
PMID: 17875928
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: classical fluorescence spectroscopy, detection by mass spectrometry, mutation analysis, protein kinase assay, western blot. ELM ID: ELMI001447; ELM sequence: KPLSPDA; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: