DmeEX0001929 @ dm6
Exon Skipping
Gene
FBgn0250819 | CG33521
Description
This gene is referred to in FlyBase by the symbol DmelCG33521 (FBgn0250819). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (3 unique). Gene sequence location is 4:1185768..1192985. Its molecular function is described by: actinin binding; structural constituent of muscle. It is involved in the biological process described with: sarcomere organization; muscle tissue development; actin cytoskeleton organization. 5 alleles are reported. No phenotypic data is available. The phenotypic class of alleles includes: viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed during late pupal stages.
Coordinates
chr4:1186473-1187880:+
Coord C1 exon
chr4:1186473-1186634
Coord A exon
chr4:1187522-1187572
Coord C2 exon
chr4:1187735-1187880
Length
51 bp
Sequences
Splice sites
3' ss Seq
ACAACTCTTATATTTTCTAGAAA
3' ss Score
7.01
5' ss Seq
CATGTACGT
5' ss Score
6.32
Exon sequences
Seq C1 exon
CGACGTCTGCACTTTAAAATTTACCAGCAAGCCGTTTAAACTAACATGGACAGTCTAAACCCCCAAAGTTCAAAAATATCTTTCGGAAATGGATTACCCTTAAAAAAAAAAAAAACTAAGAAATCAAAAACAAATGAATATGATAACCAAAATATAAATATG
Seq A exon
AAAAAAAGTTTTACGAAATTCGATGATCTACAAAAACGTAATGTTATTCAT
Seq C2 exon
TCTACAATACCTGAGAAGGTTGAAAACTGCCACCAATGCAAGAAACCCGTGTACAAAATGGAGGAAGTCATTCTTAGTTTGAAGACAGCAACGACTATTTTCCATAAAACGTGCTTACGTTGCAAGGACTGTGGCAAACACCTTAA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0250819_MULTIEX1-3/3=2-C2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.582 A=0.015 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0041217=LIM=PU(65.0=79.6)
Main Inclusion Isoform:
FBpp0090950
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0090952, FBpp0090953, FBpp0306723
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGTCTAAACCCCCAAAGTTCA
R:
TGGTGGCAGTTTTCAACCTTCT
Band lengths:
148-199
Functional annotations
There are 1 annotated functions for this event
PMID: 17875928
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: classical fluorescence spectroscopy, detection by mass spectrometry, mutation analysis, protein kinase assay, western blot. ELM ID: ELMI001447; ELM sequence: KPLSPDA; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)