MmuEX6066472 @ mm10
Exon Skipping
Gene
ENSMUSG00000032289 | Thsd4
Description
thrombospondin, type I, domain containing 4 [Source:MGI Symbol;Acc:MGI:2672033]
Coordinates
chr9:60002851-60252786:-
Coord C1 exon
chr9:60252650-60252786
Coord A exon
chr9:60056851-60057055
Coord C2 exon
chr9:60002851-60003026
Length
205 bp
Sequences
Splice sites
3' ss Seq
CCTTTTCTATCTTTTTGCAGAGC
3' ss Score
12.01
5' ss Seq
TAGGTGAGT
5' ss Score
8.83
Exon sequences
Seq C1 exon
TAAAGGGTAATCGCAAGTGTGAGCTGAACTGCCAGGCAACCGGCTACCGCTTCTACGTCCGGCAAGCTGAGAAGGTCATCGATGGCACACCCTGTGATCAGAATGGCACGGCAATCTGTGTGTCGGGGCAGTGCAAG
Seq A exon
AGCATTGGCTGTGACGACTTCCTGGGATCTGACAAAGTCTTGGACAAGTGCGGGGTGTGTGGAGGTGATAACACGGGCTGCCAGGTGGTGTCGGGAGTGTTCAAGCACGCCCTCACCAGCCTGGGCTACCACCGGGTTGTAGAGATTCCCCAAGGAGCTACGAAAATTAACATTACAGAGATGCACAAGAGCAACAACTACTTAG
Seq C2 exon
CCTTGCGAAGTCGCTCTGGCCGCTCCATCATCAATGGGAACTGGGCAATCGATCGCCCGGGGAAGTACGAGGGCGGAGGGACAATGTTCACCTACAAACGACCGAACGAGGTCTCCAGCACTGCGGGGGAGTCCTTTCTGGCTGAGGGTCCCACTAATGAGATCCTGGATGTCTAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032289-'21-32,'21-30,27-32
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.390
Domain overlap (PFAM):
C1:
NO
A:
PF059869=ADAM_spacer1=PU(35.0=59.4)
C2:
PF059869=ADAM_spacer1=FE(49.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
GGGTAATCGCAAGTGTGAGCT
R:
TCATTAGTGGGACCCTCAGCC
Band lengths:
293-498
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types