MmuEX6071973 @ mm9
Exon Skipping
Gene
ENSMUSG00000028064 | Sema4a
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [Source:MGI Symbol;Acc:MGI:107560]
Coordinates
chr3:88244620-88253774:-
Coord C1 exon
chr3:88253602-88253774
Coord A exon
chr3:88251913-88252063
Coord C2 exon
chr3:88244620-88244800
Length
151 bp
Sequences
Splice sites
3' ss Seq
TCTTCTCTGGCTGTCTTCAGGCA
3' ss Score
9.62
5' ss Seq
AGTGTAAGT
5' ss Score
8.46
Exon sequences
Seq C1 exon
AACGACGTGGGCGGTGAAAAGCTGCTGCAGAAGAAGTGGACCACCTTCCTCAAAGCCCAGTTGCTCTGCGCTCAGCCAGGGCAGCTGCCATTCAACATCATCCGCCACGCGGTCCTGCTGCCCGCCGATTCTCCCTCTGTTTCCCGCATCTACGCAGTCTTTACCTCCCAGTG
Seq A exon
GCAGGTTGGCGGGACCAGGAGCTCAGCAGTCTGTGCCTTCTCTCTCACGGACATTGAGCGAGTCTTTAAAGGGAAGTACAAGGAGCTGAACAAGGAGACCTCCCGCTGGACCACTTACCGGGGCTCAGAGGTCAGCCCGAGGCCAGGCAGT
Seq C2 exon
TGCTCCATGGGCCCCTCCTCTGACAAAGCCTTGACCTTCATGAAGGACCATTTTCTGATGGATGAGCACGTGGTAGGAACACCCCTGCTGGTGAAGTCTGGTGTGGAGTACACACGGCTTGCTGTGGAGTCAGCTCGGGGCCTTGATGGGAGCAGCCATGTGGTCATGTATCTGGGTACCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028064-'31-33,'31-31,32-33=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.216 C2=0.049
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(13.7=100)
A:
PF0140314=Sema=FE(12.0=100)
C2:
PF0140314=Sema=FE(14.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCAGAAGAAGTGGACCACC
R:
ACTCCACACCAGACTTCACCA
Band lengths:
258-409
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: