Special

MmuEX6073963 @ mm10

Exon Skipping

Gene
ENSMUSG00000032826 | Ank2
Description
ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025]
Coordinates
chr3:126997853-127002776:-
Coord C1 exon
chr3:127002678-127002776
Coord A exon
chr3:126998877-126998975
Coord C2 exon
chr3:126997853-126997951
Length
99 bp
Sequences
Splice sites
3' ss Seq
GGTGCTGCCATGATGGACAGAAT
3' ss Score
0.4
5' ss Seq
GCGGTAAGG
5' ss Score
9.63
Exon sequences
Seq C1 exon
CTTGGTTACACACCTTTGATCGTGGCCTGTCACTATGGAAATGTGAAAATGGTCAACTTTCTTCTGAAACAGGGAGCAAATGTCAATGCGAAAACCAAG
Seq A exon
AATGGCTACACACCTTTGCACCAAGCTGCCCAGCAGGGCCACACACACATCATTAATGTCCTGCTCCAGCACGGGGCCAAGCCTAATGCTACTACTGCG
Seq C2 exon
AATGGTAACACGGCCCTGGCGATTGCTAAGCGGCTGGGCTACATCTCCGTGGTTGACACCCTGAAGGTTGTGACTGAAGAGGTCACCACCACTACCACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032826-'62-73,'62-70,64-73=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
Alternative protein isoforms (No Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.054 A=0.356 C2=0.121
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF127962=Ank_2=PD(44.9=93.9)

							
A:
PF127962=Ank_2=PD(33.3=93.9)

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000138251





     
                   









    
Main Skipping Isoform:
ENSMUST00000182064fB5780





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000138620, ENSMUSP00000138753, ENSMUSP00000138781
          









    
Other Skipping Isoforms:
NA
  



Associated events







Other assemblies


mm9




Conservation


Human
(hg38)
chr4:113292416-113292514 
ALTERNATIVE
HsaEX0004130
(ANK2)
Human
(hg19)
chr4:114213572-114213670 
ALTERNATIVE
HsaEX0004130
(ANK2)
Rat
(rn6)
chr2:231302457-231302555 
HIGH PSI
RnoEX6011464
(Ank2)
Cow
(bosTau6)
chr6:13475744-13475807 
Chicken
(galGal4)
chr4:56413902-56414000 
HIGH PSI
GgaEX1065928
(chAnk2)
Chicken
(galGal3)
chr4:58369078-58369176 
HIGH PSI
GgaEX1065928
(Q90716_CHICK)
Zebrafish
(danRer10)
chr7:57659008-57659106 
HIGH PSI
DreEX6007826
(ank2b)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
ATCGTGGCCTGTCACTATGGA

				
R: 
GTGGTAGTGGTGGTGACCTCT

				
Band lengths: 
179-278

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 1 annotated functions for this event 


PMID: 14722080
HsaEX0004130
Exon 21 (HsaEX0004130) encodes ankyrin repeat 23. InsP3R-Ankyrin-B Interaction Requires Tips of beta-Hairpin Loops of ANK Repeats 22-24, and therefore it is expected to be affected by skipping. The function of Hsa0004130 has not been examined in isolation.


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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