MmuEX7000878 @ mm10
Exon Skipping
Gene
ENSMUSG00000026889 | Rbm18
Description
RNA binding motif protein 18 [Source:MGI Symbol;Acc:MGI:1915139]
Coordinates
chr2:36127125-36134263:-
Coord C1 exon
chr2:36134135-36134263
Coord A exon
chr2:36129041-36129134
Coord C2 exon
chr2:36127125-36127251
Length
94 bp
Sequences
Splice sites
3' ss Seq
TTTCATTCCTTTTGTTTCAGTGT
3' ss Score
9.77
5' ss Seq
AAGGTCACA
5' ss Score
1.86
Exon sequences
Seq C1 exon
TATTTCTCAGAGATTGATGGAAACAGAAACCAAAACTCTTCCCCTGGAGAATGCATCCATCCTTTCAGAGGGCTCTCTACAAGAAGGACACCGATTATGGATTGGCAACCTGGATCCCAAAATCACAGA
Seq A exon
TGTTCCGATAATTTCGAGAAATTATCAAACAAGAAAAGAAATCTTAGAGAAGTTGAAAGACACAGACCTAAGCAGATGTTCCTGGAATCTTAAG
Seq C2 exon
ATACCACCTCCTCAAGCTCCTCCAGAAGTTTGGCAAGGTGAAGCAGTTTGACTTCCTCTTCCATAAGTCAGGTGCTTTGGAGGGCCAGCCCCGAGGGTACTGTTTTGTTAACTTTGAAACTAAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026889-'6-6,'6-5,8-6=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
In the CDS, with uncertain impact
No structure available
Features
Disorder rate (Iupred):
C1=0.149 A=0.094 C2=0.078
Domain overlap (PFAM):
C1:
PF0007617=RRM_1=PU(14.9=28.9)
A:
NO
C2:
PF0007617=RRM_1=FE(56.8=100)
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCCCTGGAGAATGCATCCAT
R:
CCCTCCAAAGCACCTGACTTA
Band lengths:
174-268
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.847, FDR=0.069), PC9 Enriched at 14 days (FC=1.273, FDR=0.005), Late Xenograft No change (FC=1.077, FDR=0.907).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types