DmeEX6002349 @ dm6
Exon Skipping
Gene
FBgn0030571 | CG14414
Description
This gene is referred to in FlyBase by the symbol DmelCG14414 (FBgn0030571). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (3 unique). Gene sequence location is X:14580600..14582174. Its molecular function is described by: mRNA binding; RNA binding. The biological processes in which it is involved are not known. 7 alleles are reported. The phenotype of these alleles manifest in: trichogen cell. The phenotypic classes of alleles include: fertile; short lived; viable; visible. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes.
Coordinates
chrX:14580965-14582174:+
Coord C1 exon
chrX:14580965-14581039
Coord A exon
chrX:14581582-14581674
Coord C2 exon
chrX:14581749-14582174
Length
93 bp
Sequences
Splice sites
3' ss Seq
ATCCCAAAAATTCACTCCAGAAC
3' ss Score
4.01
5' ss Seq
TATGTAAGC
5' ss Score
4.2
Exon sequences
Seq C1 exon
TTCGACATGCTGTTCCACAAGGGCGGTCCCATGGTGGGACAATCCAGGGGCTACGCCTTCGTCACCTTTGCGCAG
Seq A exon
AACGAGGGAGCCACCAATGCGCTACTGAAACTGGACGGAACCAGCGTGGGAAATCGCTCGATAGCCGTGCGTTTGGCCAAGAACATTAAATAT
Seq C2 exon
GATGAAACGCAGAAGCCAAAGCCGCGCCTGGATATACCGGCCTTGGGCACAGGTAAACGCGAGGAGAAGGTCAGCAAAACGGAGGCCATTCGGGCCATTGAGGCCAAGCTGAAAATGTTGGAGCGCCAGACAGACGACAATCTCGAGCTGAATACAGCGGGCAGGGGCGAGGCCAACGTGCCCTTCATCCAGCGCTATCAGTTTAACAAGGATCGCGACGGTTCGCAGCGCTACGGAAAGTCATCGGCTCCATATCATCACCAGCAGCGTCCCAAGCGCCACTAGGCTCAATCGACCCACACTCGACTTTGCCACCGTCTCACGCATTAGTCTGTAAAAATCATTAGTACTTAGGGCGCTACCTCAGCAGACGAGCCTTGCAGGTGTAATTTACAATTAATTTATTAAAAAAAAAGGTACAATCAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0030571-'2-7,'2-6,3-7=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.026 A=0.137 C2=0.742
Domain overlap (PFAM):
C1:
PF0007617=RRM_1=PU(41.0=69.6)
A:
PF0007617=RRM_1=PD(53.8=67.7)
C2:
NO
Main Inclusion Isoform:
FBpp0308546
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0073747, FBpp0073749, FBpp0292380
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
TGGGACAATCCAGGGGCTAC
R:
TGTCTGGCGCTCCAACATTTT
Band lengths:
173-266
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.847, FDR=0.069), PC9 Enriched at 14 days (FC=1.273, FDR=0.005), Late Xenograft No change (FC=1.077, FDR=0.907).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)