MmuINT0059588 @ mm10
Intron Retention
Gene
ENSMUSG00000001815 | Evx2
Description
even-skipped homeobox 2 [Source:MGI Symbol;Acc:MGI:95462]
Coordinates
chr2:74657767-74659557:-
Coord C1 exon
chr2:74658993-74659557
Coord A exon
chr2:74658048-74658992
Coord C2 exon
chr2:74657767-74658047
Length
945 bp
Sequences
Splice sites
5' ss Seq
AAGGTAACC
5' ss Score
9.24
3' ss Seq
GTGAACTGTTTGTTTGCTAGGGT
3' ss Score
7.8
Exon sequences
Seq C1 exon
CAATTTTTTCCGCCTCCCTCCAAAAGAGATAATAATATTTAGAGCCGCTTGCTGGGGCTGAATGAGAATCAGCCCTGGGCGCAGCCCATCATTAAGACGGGACAGCCAGGCCACTGTGACATTTTTTAGAAAGCTGAGATGATGGAAAGAATAAGAAAAGAGATGATTCTGATGGAAAGAGGGCTCCACAGCCCTACTGCTGGCAAGAGGTTCTCCAGTTTGTCCGATTCAGCTGGCGGTGCTGTGCTCGAGGCCCTGGAAAATTCGCAGCACCCGGCTCGCCTCAGTCCGCGCCTGCCGTCCGCCCCGCTGCACGGAGCTCTGGGAGACCTCCCCGCCAAAGGCAAATTCGAAATAGACACTTTGTTCAACCTGCAGCACCCGAGCAGCGAAAGCACCGTCTCCTCCGAAATTGCCTCCGCTACCGAGAGCCGCAAGAAGCCTAGTCATTATTCCGAAGCGGCCGCCGAGGCCGACATGAGCAGCGACGTGGAGGTGGGCTGCTCCGCACTGCGCTCCCCCAGCGGCCTGGGCGCTGCACCGCTCAAGGAAAACAATGCCAAAG
Seq A exon
GTAACCGGGTACCCGCAGACTCCAGCCTCATCCTCTGGGCTCTCTCGAGGGCCTGGCCAGCCCTTTCCACACCTTAGCGGGTCTCTGACTCTTAACTAAAGTGCTTCACAGAGCTGGCAGGGGCAGGGGAGGGATGCATACTAAAAACTGGTCCCTCTGGCTCTGTTCTTTGAGCATCCCAGCACAGCCCGGGCCTGTGGAGCCTAGGGAGCCAGGCCCAAGCGCCCAGGCTCCTGCCTGAATCGAAGCAAATGTTTCGCTTGCCGATCTAAGAGCTGGGGCGTAAGAGCTGGGGCGAGGCGACACAGCGCTGCTAGCCCGGGTGGCTTAGTTGCTTTTTGGGAAGGGTGCCCTGGACAGGAGCTCCGAACTCAGTTGCTTCTGGAGCCCCCAGGGCCGGGTAGAGGGGACACTCCTCTGGATAAGTCTGAGACAGAGAGTCTGGCCTCGCACCTCTGCTCAACCGCTTAAGTCAGCGGACAGAAAAGAGTTTGAAAAGTGGGCGCCTCCTTCCCTTTACTGGCTTGAGCTTGAGCTGCAAATGCTTTGAAAATCCTAAGATATTTTAACCCCAAAGGCTGTCAAAGAAAGATTGGAAGCCGCATAAAGAGCTGCCCTATGGTTCCTAGACTGTCAAACCCACAGCTTCTTAAACTGGTTCCAAGGTAGGCATTCTTCCCCAGCAAATTTTCCTACCGAGTGGTAATTTTCTAATTATTGGAAAAAGAACAACCTTTTCAAATTCTGTTTCTATTATTTGGTTAGGAAGGGCTCCCCAAACTTCATTGATTTCATTAACGGCAGCAATTTGTAAGCCTGACAGATCCCATCAATTTTGACAGCGACTTTCCTTCTCAAAGCCTCTTTTGCCACCAGCTAGCAACCCCAACTCAGGGTTCTCGGTCCCTACAACTACTCATGAGCTGTGAACTGTTTGTTTGCTAG
Seq C2 exon
GGTACACGGAGAGCGGCTCCGTCGCGGGCACCACGACGTCGGCTTCCGGCTCGGGCCTCGGTAGTCTGCATGGAGGCGGCGGCGGCGGCAATAGCGGGGCTGCGGCGTTGGGCGGCTCTGGCTCCGGTTCCGGCGCCGATCAGGTGCGGCGATACCGTACGGCATTCACCCGCGAACAGATCGCGCGCCTGGAGAAAGAGTTCTACAGGGAGAACTACGTGTCTCGGCCCCGCCGGTGCGAGCTGGCCGCTGCACTCAACCTGCCCGAAACCACCATCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000001815:ENSMUST00000001867:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.780 A=NA C2=0.564
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0004624=Homeobox=PU(77.2=46.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCACCGTCTCCTCCGAAATTG
R:
GGGTGAATGCCGTACGGTATC
Band lengths:
342-1287
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types