MmuINT0059588 @ mm9
Intron Retention
Gene
ENSMUSG00000001815 | Evx2
Description
even skipped homeotic gene 2 homolog [Source:MGI Symbol;Acc:MGI:95462]
Coordinates
chr2:74495824-74497476:-
Coord C1 exon
chr2:74497050-74497476
Coord A exon
chr2:74496105-74497049
Coord C2 exon
chr2:74495824-74496104
Length
945 bp
Sequences
Splice sites
5' ss Seq
AAGGTAACC
5' ss Score
9.24
3' ss Seq
GTGAACTGTTTGTTTGCTAGGGT
3' ss Score
7.8
Exon sequences
Seq C1 exon
ATGATGGAAAGAATAAGAAAAGAGATGATTCTGATGGAAAGAGGGCTCCACAGCCCTACTGCTGGCAAGAGGTTCTCCAGTTTGTCCGATTCAGCTGGCGGTGCTGTGCTCGAGGCCCTGGAAAATTCGCAGCACCCGGCTCGCCTCAGTCCGCGCCTGCCGTCCGCCCCGCTGCACGGAGCTCTGGGAGACCTCCCCGCCAAAGGCAAATTCGAAATAGACACTTTGTTCAACCTGCAGCACCCGAGCAGCGAAAGCACCGTCTCCTCCGAAATTGCCTCCGCTACCGAGAGCCGCAAGAAGCCTAGTCATTATTCCGAAGCGGCCGCCGAGGCCGACATGAGCAGCGACGTGGAGGTGGGCTGCTCCGCACTGCGCTCCCCCAGCGGCCTGGGCGCTGCACCGCTCAAGGAAAACAATGCCAAAG
Seq A exon
GTAACCGGGTACCCGCAGACTCCAGCCTCATCCTCTGGGCTCTCTCGAGGGCCTGGCCAGCCCTTTCCACACCTTAGCGGGTCTCTGACTCTTAACTAAAGTGCTTCACAGAGCTGGCAGGGGCAGGGGAGGGATGCATACTAAAAACTGGTCCCTCTGGCTCTGTTCTTTGAGCATCCCAGCACAGCCCGGGCCTGTGGAGCCTAGGGAGCCAGGCCCAAGCGCCCAGGCTCCTGCCTGAATCGAAGCAAATGTTTCGCTTGCCGATCTAAGAGCTGGGGCGTAAGAGCTGGGGCGAGGCGACACAGCGCTGCTAGCCCGGGTGGCTTAGTTGCTTTTTGGGAAGGGTGCCCTGGACAGGAGCTCCGAACTCAGTTGCTTCTGGAGCCCCCAGGGCCGGGTAGAGGGGACACTCCTCTGGATAAGTCTGAGACAGAGAGTCTGGCCTCGCACCTCTGCTCAACCGCTTAAGTCAGCGGACAGAAAAGAGTTTGAAAAGTGGGCGCCTCCTTCCCTTTACTGGCTTGAGCTTGAGCTGCAAATGCTTTGAAAATCCTAAGATATTTTAACCCCAAAGGCTGTCAAAGAAAGATTGGAAGCCGCATAAAGAGCTGCCCTATGGTTCCTAGACTGTCAAACCCACAGCTTCTTAAACTGGTTCCAAGGTAGGCATTCTTCCCCAGCAAATTTTCCTACCGAGTGGTAATTTTCTAATTATTGGAAAAAGAACAACCTTTTCAAATTCTGTTTCTATTATTTGGTTAGGAAGGGCTCCCCAAACTTCATTGATTTCATTAACGGCAGCAATTTGTAAGCCTGACAGATCCCATCAATTTTGACAGCGACTTTCCTTCTCAAAGCCTCTTTTGCCACCAGCTAGCAACCCCAACTCAGGGTTCTCGGTCCCTACAACTACTCATGAGCTGTGAACTGTTTGTTTGCTAG
Seq C2 exon
GGTACACGGAGAGCGGCTCCGTCGCGGGCACCACGACGTCGGCTTCCGGCTCGGGCCTCGGTAGTCTGCATGGAGGCGGCGGCGGCGGCAATAGCGGGGCTGCGGCGTTGGGCGGCTCTGGCTCCGGTTCCGGCGCCGATCAGGTGCGGCGATACCGTACGGCATTCACCCGCGAACAGATCGCGCGCCTGGAGAAAGAGTTCTACAGGGAGAACTACGTGTCTCGGCCCCGCCGGTGCGAGCTGGCCGCTGCACTCAACCTGCCCGAAACCACCATCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000001815-Evx2:NM_007967:1
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.776 A=NA C2=0.564
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0004624=Homeobox=PU(77.2=46.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCACCGTCTCCTCCGAAATTG
R:
GGGTGAATGCCGTACGGTATC
Band lengths:
342-1287
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: