MmuINT0094975 @ mm9
Intron Retention
Gene
ENSMUSG00000018932 | Map2k3
Description
mitogen-activated protein kinase kinase 3 [Source:MGI Symbol;Acc:MGI:1346868]
Coordinates
chr11:60756653-60757108:+
Coord C1 exon
chr11:60756653-60756766
Coord A exon
chr11:60756767-60756988
Coord C2 exon
chr11:60756989-60757108
Length
222 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAGC
5' ss Score
10.22
3' ss Seq
GACATGTGGCTCTCCTATAGCGC
3' ss Score
6.78
Exon sequences
Seq C1 exon
AACTTCGAAGTGGAGGCTGATGACTTGGTGACCATCTCAGAGCTGGGTCGTGGAGCCTATGGGGTGGTAGAGAAAGTGCGGCATGCTCAGAGTGGTACCATCATGGCTGTCAAG
Seq A exon
GTAAGCAGGGCTTCACCCTACCTGGAGTAGCACTGGAGGAGGCACTGGCTGAGCTTCAGAGTGACTGTCTACTTGTGGCTCTGTCCCTCACTGTCATCACCAGCGTTGAGGCAGAACTTCTCAGACAAGTCAGGGCCATGGATTCCCCAAGGTTCCTGGCCCTCATGGACTGGGTTAGGGGAACAGCCTAATCCGTGGCCCTGACATGTGGCTCTCCTATAG
Seq C2 exon
CGCATCCGGGCCACAGTGAACACACAGGAGCAGAAACGTCTGCTTATGGACCTAGACATCAACATGCGCACGGTCGACTGCTTCTACACTGTCACCTTCTATGGTGCCCTCTTCAGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000018932-Map2k3:NM_008928:4
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=PU(11.1=76.3)
A:
NA
C2:
PF0006920=Pkinase=FE(14.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTTCGAAGTGGAGGCTGATG
R:
GAAGGTGACAGTGTAGAAGCAGT
Band lengths:
212-434
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: