MmuINT0097664 @ mm9
Intron Retention
Gene
ENSMUSG00000057751 | Megf6
Description
multiple EGF-like-domains 6 [Source:MGI Symbol;Acc:MGI:1919351]
Coordinates
chr4:153636623-153637410:+
Coord C1 exon
chr4:153636623-153636748
Coord A exon
chr4:153636749-153637278
Coord C2 exon
chr4:153637279-153637410
Length
530 bp
Sequences
Splice sites
5' ss Seq
CCGGTGAGT
5' ss Score
10.9
3' ss Seq
ACCCTGGGTGTGGGTTCTAGACT
3' ss Score
6.15
Exon sequences
Seq C1 exon
AGTGCCCAGTGGGGACATTTGGTGTGAACTGCTCAGGTTCCTGCTCCTGCGTGGGGGCCCCCTGTCACCGAGTCACGGGGGAGTGTCTGTGCCCACCAGGGAAGACTGGGGAGGACTGTGGAGCCG
Seq A exon
GTGAGTGGCATCTGTATGCCCAGGCAGCCCCAGCCCTGGGCTGTGTTTGAAAATACCACCCCGGACCATATGTAGGGACCCCTGATCCCAGATCTGGACTGCTTGGGAACCCCACCATAGACCATTTAGGACCTTAGCCATGACCCTGAAGTGTTAGGTTCCTTCATAGTAGCTTCGGGGAGTCGTCCTGGAACAAGCGGGGATATGGGAGGTGTCTGCAGCTGCTCAGCAGAGCTGTGACTTTGCACTGAGCCCTCCTGGACCGTCAGCTCCCTGCATGGGGTATTTGTCTCTCTAGAAGGCTCTCTATTTTGCTGTTGAGCTATCCCTGGGGTTAGGTTTACTTATTTATAGGTGGTGCCCTGCAGCTCTGGGGTTCTGAAACCGGACTCTCCTGGGCAGTCTTTTATGGCAACAGTGGTCTGTTTCCAGTTGTGGCAAGAGGGACCTTTAGTGCTGTGTCGGACGCACACCAGCGTGCCAACTAGGGCACTAGGAAAAGAATAACTAACCCTGGGTGTGGGTTCTAG
Seq C2 exon
ACTGTCCCGAGGGCCGTTGGGGGCTCGGCTGTCAAGAGATCTGCCCTGCATGTGAGCATGGTGCCAGCTGCGACCCTGAGACTGGAACCTGCCTGTGTCTTCCTGGTTTTGTTGGTAGCCGCTGCCAGGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057751-Megf6:NM_001162977:18
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0005319=Laminin_EGF=PU(68.9=68.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAGTGGGGACATTTGGTGTG
R:
GCAGCGGCTACCAACAAAAC
Band lengths:
246-776
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: