MmuINT0097689 @ mm9
Intron Retention
Gene
ENSMUSG00000057751 | Megf6
Description
multiple EGF-like-domains 6 [Source:MGI Symbol;Acc:MGI:1919351]
Coordinates
chr4:153626238-153626670:+
Coord C1 exon
chr4:153626238-153626360
Coord A exon
chr4:153626361-153626532
Coord C2 exon
chr4:153626533-153626670
Length
172 bp
Sequences
Splice sites
5' ss Seq
ACCGTGAGT
5' ss Score
9.4
3' ss Seq
CAGCTGGGATCTCCTCACAGGGA
3' ss Score
5.74
Exon sequences
Seq C1 exon
ATGTGGACGAATGTGCCTTGGGTCTGGCCCAGTGTGCTCATGGCTGCCTCAACACTCAGGGGTCCTTTAAGTGTGTGTGTCACGCAGGCTATGAGCTGGGTGCTGATGGCCGGCAGTGTTACC
Seq A exon
GTGAGTCACCAGGACTGGGGGTTACCGCAAACAGTGGACTGGGGGTGATTGGGGTACTAATGTGGCTCCCCAAGACCCTAGAGTCAGTTTCAGTTGGGCAGCTCTGGGTGCGGGTCCCCAGGGGCTGCTGAGGGAGTGCCGACCCCTCATCTCAGCTGGGATCTCCTCACAG
Seq C2 exon
GGATCGAGATGGAGATTGTGAACAGCTGCGAGGCTGGCAACGGAGGCTGCTCACACGGCTGTAGTCACACTAGCACAGGGCCCCTGTGCACCTGCCCTCGTGGTTATGAGCTGGATGAGGACCAGAAGACCTGCATTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057751-Megf6:NM_001162977:8
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0764510=EGF_CA=WD(100=95.2)
A:
NA
C2:
PF146701=FXa_inhibition=WD(100=76.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGTGGACGAATGTGCCTTGG
R:
TGCAGGTCTTCTGGTCCTCAT
Band lengths:
258-430
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: