Special

MmuINT0100294 @ mm9

Intron Retention

Gene
ENSMUSG00000079184 | Mphosph8
Description
M-phase phosphoprotein 8 [Source:MGI Symbol;Acc:MGI:1922589]
Coordinates
chr14:57292728-57295455:+
Coord C1 exon
chr14:57292728-57293576
Coord A exon
chr14:57293577-57295358
Coord C2 exon
chr14:57295359-57295455
Length
1782 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAAG
5' ss Score
8.27
3' ss Seq
TTGCATTCATTATTCTTTAGGAG
3' ss Score
7.54
Exon sequences
Seq C1 exon
AGACTATCTTTAAACAATGACATATTTGAGGCAGACAGTGATAGCGACCAGCAAAGTGACACAAAAGAAGATATTTCACCAAGGAAGAAAAAGAAAAAAATAAAGTGCAAAGAAGAGACAAGTCCAGAAGATCTGAGGAAAAAAAGGACAAAAATGGGAAAACTAAAAGACAAGTTCAAGACAGAGCTGGAGAGCACCTCTGAAATAATCGGTTTCGATGTAAAGACGAAGAAAAGAATTTGGGAAGTCAAGGAAGAATTAAAGGACTCCAAAAAGCCCAAAAAGGATGAAATAAAGGAAACCAAGGAATTAAAGAAAGCTAATAAAAGGGCTGAAGTAAGAGATTTAAAGATTAAAATAAGAGAAGATGTCAAAGAGAACCGAAAAACAAAAAAAGAGAGATATATAGAATCTCCGCTGGAGTCTGAATCGCCTAATGATTCCCTCATTCTAGAGGATGACAGTGAAGACTTTATTTCTGACAACAGAGAAGAGAATCAGAATGTGAGAAGTGTAAGAGATAAGACAGCACAGGAGACAGTGCAAGAGGGTATTTTTGAGAAACATCTGGATGACCTTATAAGTATTGAAGAGGATGCTGGTACCCGAGTTAGAAGGAAGAAGACGAAGCCAAGAAAGTTTGAGGAACCAAAAGAGATCAAGAAGCTTGAAAGCACGAATGCCTTTTTAGAAAGGAGAGCAATACCCAAAAAGCAAAGAAACCAAGACAAGGGCATAAGTAACCTAGAGTTAAATAAGCTACCATCACCTGTGTTTGCCCAAACCCTGAAAAGTTCCAGACTGAGTGGGGAAGAGAAGAGCCTAAAGTCCCCTGACTTGGCAGAAGAG
Seq A exon
GTAAAGTTGGTGGAGAACTTGAAGAGTACAGATATGGTTGGTACAGATTATTACTTGCTGTTGTATGGTTAGGAACTTTCACAAGGAATGACAAAGGAAACAGTCAAACTAGAATAAAGTGTAACTCAGGTAGTTATTAAATATGGTGGCACTGAAGTTGCCTGTGTGTGCTCAACTATTAAGCACCGGAACCCCAAGTTTACCTTTCAGTTTGTGCCTTGTTTTTTTTTTTTTTAATATTTTTTATTACGTATTTTCCTCAATTACATTTGTGCCTTGTTTTTTGTTTGTTTGTTTTACCAAACAAGACAAACCCCTGAGAGTTAATTTGCTACCAAAGTCAGAATTTTTGTAGATTTGTGCAGCATTTTATAGTCAGGCCTATCAGCTTCATCATCTTACTAATTTCATTTGGGGTATGTATGTTCTCTATTTTAAAAATACTGAAGTTTGATACAGTGCACTTTGATCTTATTCCCCTCTACTCTTTCCCTGACTTCCTCCAGATCCACTTTTACCTCTCCTTTCACCCAGCTTGTGGGGTTTGGTTTGGTTTGGTTTGGTTTTAGCTTTATCATGTCTAGTTTGTATTGTCCATATACTGAATGTGGGGCCATCTTCAGGTACATGGTAGATTTACTAGTAGCCACACACACCCTTAAAGAAAACCTAGAAACTACCCAATAGTCAGCTCCATAGCTCTCCTCATTTAGGAGTGGAACTTAGTGGCTCCTTTCCTGTTTCATGTTAAAATGTTGACGAGCTTGATCTTGTGTCTATCATACTTCCTCCCTGCCCCTCTCTAACAAAATGTCTTACACTATAGCCCAGGCTAGCCTTGAGCTGGTCCTCTGGCTCCTCCTTCCTGGGTGGGAGTTGAAGTTACGTTTGGCTTTCACTTTATTTGGGGGAGGGCGGTGATACGATTTATTTGTATGGCTTGGCAGTAGTAGTACCTGGTGGTAGTACCCACCTTTAATCCAGTGCTCAGGAGGCAGAGGCAGGTGGATCTCTGAGTTCAAAGCTAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTTCACTAGAGAAACCTGTCTTAAAAAACAAACAAATGCACAAAAACAATAAAAGATTTCTAGATTTATTGGTATGAATGTTTTATTTGCATATAGGTACACGTATCACGTGTGCCTGGTGCCCAGAGAGTCCAGAAAAAGGCATCATATAGTCATTGAGTGCTGGGAACTGAACCCAGGTTCTTTATGAGAGCACCAAGTACTCTTAACATCTGTGCCATCACATCACTGCAGCCCCAAGATTGTTTTTATAGAGTTTAATTTTGTAGCAATTTAGTTTTTATTATACAAAAGAAGGGCTTTCACGGTGACATTTTCATACCTGTATATAATGAATTGCAAGGTTTTTTTTGTTGTTGTTGTTGTTTTATTTGAAATAAGGTCTCACTCCATGACTGAGCTGGCCTTGAATTTATGTAGATCTGTCCCTGCCATCCTAGTGCTAAGATTAAAGGTTTACACTATCATGTAAATGTATATTCAAACATTTTAAGTAATAGATTCTTAAAAGAAAATGGAGAAACAAAGTATTGGGTTTTGGGTTTCATTTCATATGGCTTAGCGTGTCTGCAGAATAGTCTTGGTGGCGGGAGGGAGCAGTGGGAGAAACATTAAATAATCAGCTGTGTGCTCAGGCGTGTGTAGAATATTTGGTTTGCAAATATTACAGTATCAACTCTTTATCTTTTATTTTTTGTTTTGCATTCATTATTCTTTAG
Seq C2 exon
GAGAAAGAGAAAAAAAATGAACCCAAAGGAAAATACCAGAAAAGGTATGATTTGGACAAAGAAGAAAAAGCCAGAAAAGAGCCAAAGGTATTAAAAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000079184-Mphosph8:NM_023773:3
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.714 A=NA C2=0.877
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NA

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000112170





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000112170f5550A
  



Associated events







Conservation


Human
(hg38)
No conservation detected
Human
(hg19)
chr13:20221432-20222561 
HsaINT0104635
(MPHOSPH8)
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
chr12:36622748-36626585 
ALTERNATIVE
BtaINT0094533
(MPHOSPH8)
Chicken
(galGal4)
chr1:178481263-178481384 
Chicken
(galGal3)
chr1:183537976-183538758 
GgaINT0112888
(MPHOSPH8)
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GAAGAGGATGCTGGTACCCGA

				
R: 
ACCTTTGGCTCTTTTCTGGCT

				
Band lengths: 
350-2132

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






Retrieved from "https://vastdb.crg.eu/wiki/Special:VastDB"